Increased colonic apelin production in rodents with experimental colitis and in humans with IBD
Increased colonic apelin production in rodents with experimental colitis and in humans with IBD
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DOI：
10.1016/j.regpep.2007.02.002
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发表时间：
2007-08-16
影响因子：
--
通讯作者：
Greeley, George H., Jr.
中科院分区：
文献类型：
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作者：
Han, Song;Wang, Guiyun;Greeley, George H., Jr.
爱帕琳及其受体（AN受体）在胃肠道中表达。本研究的目的是检查葡聚糖硫酸钠（DSS）诱导的大鼠和小鼠实验性结肠炎和人类炎症性肠病（IBD）对肠道apelin产生的影响，以及外源性apelin对小鼠结肠上皮细胞增殖的影响。在患有实验性结肠炎的啮齿动物中，结肠爱帕琳mRNA水平在炎症反应期间以及在DSS停药后的组织修复阶段期间升高。结肠爱帕琳蛋白表达的波动被爱帕琳蛋白免疫染色的类似变化所掩盖。Apelin免疫染色在表面上皮、沿管状腺长度沿着的上皮细胞和腺基部的干细胞区域中增加。在溃疡性结肠炎（UC）和克罗恩病患者中，爱帕琳免疫染色显示肠爱帕琳含量增加的模式与在患有实验性结肠炎的啮齿动物中观察到的相似。在DSS诱导的结肠炎的恢复期向小鼠施用合成的爱帕琳显著刺激结肠上皮细胞增殖。我们观察到结肠爱帕琳肽的产生在DSS暴露期间和之后增加，表明爱帕琳肽在结肠炎的不同阶段发挥多种作用。此外，外源性爱帕琳对结肠上皮增殖的刺激作用表明，在肠恢复阶段期间增加的爱帕琳产生可能有助于结肠炎的实验啮齿动物模型和IBD患者中肠上皮的修复。(C)2007 Elsevier B.V.保留所有权利。
Apelin and its receptor, the AN receptor, are expressed in the gastrointestinal tract. The aims of this study were to examine the effects of sodium dextran sulfate (DSS)-induced experimental colitis in rats and mice and inflammatory bowel disease (IBD) in humans on intestinal apelin production, and the influence of exogenous apelin on colonic epithelial cell proliferation in mice. In rodents with experimental colitis, colonic apelin mRNA levels were elevated during the inflammatory reaction as well as during the tissue repair phase that ensues after DSS withdrawal. Fluctuations in colonic apelin expression were paralleled by similar changes in apelin immunostaining. Apelin immunostaining was increased in the surface epithelium, in epithelial cells along the length of the tubular gland and in the stem cell region at the gland base. In ulcerative colitis (UC) and Crohn's disease patients, apelin immunostaining revealed a pattern of increased intestinal apelin content similar to that observed in rodents with experimental colitis. Administration of synthetic apelin to mice during the recovery phase of DSS-induced colitis stimulated colonic epithelial cell proliferation significantly. Our observations that colonic apelin production is increased during and after DSS exposure indicate that apelin plays multiple roles during the different stages of colitis. Additionally, the stimulatory action of exogenous apelin on colonic epithelial proliferation suggests that the increased apelin production during intestinal recovery stage may contribute to the repair of the intestinal epithelium in experimental rodent models of colitis and in IBD patients. (C) 2007 Elsevier B.V. All rights reserved.