The tracheae defective gene encodes a bZIP protein that controls tracheal cell movement during Drosophila embryogenesis
The tracheae defective gene encodes a bZIP protein that controls tracheal cell movement during Drosophila embryogenesis
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DOI：
10.1093/emboj/16.23.7156
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发表时间：
1997-12-01
期刊：
影响因子：
11.4
通讯作者：
Schuh, R
中科院分区：
文献类型：
--
作者：
Eulenberg, KG;Schuh, R
气管缺陷基因（tdf）是果蝇胚胎发育过程中气管系统形成所必需的基因，它编码一个假定的bZIP转录因子（TDF），针对TDF的抗体可在内陷前和整个气管系统形态发生过程中检测到所有气管细胞中的一种核蛋白，对TDF突变体的检测显示TDF活性对于确定气管细胞的身份不是必需的，但是对于随后的形态发生细胞的识别是必需的。动作tdf活性受气管发育的关键调节基因trachealess的控制。相反，tdf活性不依赖于也不干扰成纤维细胞生长因子（FGF）和十肢麻痹（DPP）介导的直接引导气管细胞迁移的信号传导。我们的结果表明，缺乏tdf活性影响气管细胞迁移的一般性而不是细胞迁移的特定方面。TDF活性涉及母体和合子成分，并且其要求不限于气管系统形成。胚胎中TDF表达的复杂时空模式对应于缺陷，表明细胞迁移受损。我们提出，bZIP蛋白TDF的功能作为一个辅助调节的靶基因，提供细胞迁移的能力。
The tracheae defective (tdf) gene is required for the formation of the tracheal system during Drosophila embryogenesis, It encodes a putative bZIP transcription factor (TDF), Antibodies directed against TDF detect a nuclear protein in all tracheal cells before invagination and throughout tracheal system morphogenesis, Examination of tdf mutants revealed that tdf activity is not necessary for determining tracheal cell identity but for subsequent morphogenetic cell movements. tdf activity is under the control of trachealess, the key regulator gene for tracheal development, In contrast, tdf activity is not dependent on and does not interfere with the fibroblast growth factor-(FGF) and Decapentaplegic-(DPP) mediated signalling that direct guided tracheal cell migration, Our results suggest that lack of tdf activity affects tracheal cell migration in general rather than specific aspects of cell migration. tdf activity involves a maternal and zygotic component and its requirement is not limited to tracheal system formation. The complex spatiotemporal patterns of TDF expression in the embryo correspond to defects, suggesting that cell migration is impaired. We propose that the bZIP protein TDF functions as a co-regulator of target genes that provide cells with the ability to migrate.