Immunomodulatory effect of mesenchymal stem cells: Cell origin and cell quality variations
Immunomodulatory effect of mesenchymal stem cells: Cell origin and cell quality variations
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DOI：
10.1007/s11033-018-04582-w
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发表时间：
2019-02-01
影响因子：
2.8
通讯作者：
Li, Tao-Sheng
中科院分区：
文献类型：
--
作者：
El-Sayed, Marwa;El-Feky, Mohamed Ali;Li, Tao-Sheng
间充质干细胞（MSCs）的免疫调节特性以前已被报道。目前尚不清楚这种特性是否会受到细胞来源和细胞质量的影响。使用从小鼠骨髓（BM-MSCs）和脂肪组织（AD-MSCs）扩增的原代MSCs，我们研究了MSCs的免疫调节性质是否随细胞来源和细胞质量（早期与晚期传代的BM-MSCs）而变化。BM-MSCs（p1）和AD-MSCs（p1）具有典型的梭形细胞形态，但晚期传代的BM-MSCs（p6）出现形态学改变。聚焦于通路的阵列显示，趋化因子/细胞因子基因的表达随不同的细胞来源和质量而变化。通过与脾单个核细胞（MNC）共培养3天，所有类型的MSC均抑制CD 4的表达。相反，BM-MSCs抑制CD 8的表达，AD-MSCs增加CD 8的表达。与AD-MSCs和BM-MSCs共培养后，CD 206/CD 86的表达比例相当，但与晚期传代的BM-MSCs共培养后，CD 206/CD 86的表达比例较低。AD-MSCs在培养液中高度诱导IL-6、IL-10和TGF-β的释放。与早期传代的BM-MSCs（p1）相比，晚期传代的BM-MSCs（p6）释放较少的TGF-β。我们的数据表明，骨髓间充质干细胞的免疫调节特性随细胞来源和细胞质量而变化，高质量的骨髓间充质干细胞可能是免疫调节的最佳来源。
The immunomodulatory property of mesenchymal stem cells (MSCs) has been previously reported. Still it is unclear if this property can be affected by the cell origin and cell quality. Using primary MSCs expanded from bone marrow (BM-MSCs) and adipose tissue (AD-MSCs) of mice, we investigated whether the immunomodulatory property of MSCs varied with cell origin and cell quality (early- vs. late-passaged BM-MSCs). BM-MSCs (p1) and AD-MSCs (p1) had a typical spindle shape, but morphological changes were observed in late-passaged BM-MSCs (p6). A pathway-focused array showed that the expression of chemokine/cytokine genes varied with different cell origins and qualities. By co-culturing with spleen mononuclear cells (MNC) for 3 days, the expression of CD4 was suppressed by all types of MSCs. By contrast, the expression of CD8 was suppressed by BM-MSCs and increased by AD-MSCs. The expression ratio of CD206 to CD86 was at a comparable level after co-culture with AD-MSCs and BM-MSCs, but was lower with late-passaged BM-MSCs. AD-MSCs highly induced the release of IL6, IL-10 and TGF-beta in culture medium. Compared with early-passaged BM-MSCs (p1), late-passaged BM-MSCs (p6) released less TGF-beta. Our data suggests that the immunomodulatory properties of MSCs vary with cell origin and cell quality and that BM-MSCs of good quality are likely the optimal source of immunomodulation.