Library generation by gene shuffling.
Library generation by gene shuffling.
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DOI：
10.1002/0471142727.mb1512s105
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发表时间：
2014-01-06
影响因子：
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通讯作者：
Ellington, Andrew D
中科院分区：
文献类型：
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作者：
Meyer, Adam J;Ellefson, Jared W;Ellington, Andrew D
本单元描述了基因改组的过程，也称为有性 PCR。基因改组是一种生成包含相关基因家族信息的序列库的简便方法。本质上，相关基因通过 DNase I 消化而片段化，并通过无引物 PCR 重新组装。然后可以针对所需功能筛选或选择所得嵌合基因。
This unit describes the process of gene shuffling, also known as sexual PCR. Gene shuffling is a facile method for the generation of sequence libraries containing the information from a family of related genes. Essentially, related genes are fragmented by DNase I digestion and reassembled by primer-less PCR. The resulting chimeric genes can then be screened or selected for a desired function.