Comparison of radioimmunoprecipitation with luciferase immunoprecipitation for autoantibodies to GAD65 and IA-2beta.
Comparison of radioimmunoprecipitation with luciferase immunoprecipitation for autoantibodies to GAD65 and IA-2beta.
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DOI：
10.2337/dc09-1938
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发表时间：
2010-04
期刊：
影响因子：
16.2
通讯作者：
Iadarola MJ
中科院分区：
文献类型：
--
作者：
Burbelo PD;Hirai H;Issa AT;Kingman A;Lernmark A;Ivarsson SA;Notkins AL;Iadarola MJ
比较荧光素酶免疫沉淀法（LIPS）和放射免疫沉淀法（RIP）检测1型糖尿病自身抗原谷氨酸脱羧酶65（GAD 65）和胰岛素瘤相关蛋白（IA）-2β自身抗体的灵敏度和特异性。来自2007年糖尿病抗体标准化项目的49名1型糖尿病患者和100名非糖尿病对照受试者的血清用于筛查GAD 65的自身抗体。另外200例1型糖尿病患者和200例非糖尿病对照受试者用于验证GAD 65结果并筛查IA-2β自身抗体。LIPS检测GAD 65和IA-2β自身抗体的敏感性和特异性与RIP相同。受试者操作特征分析显示LIPS和RIP检测GAD 65和IA-2β自身抗体的差异无统计学意义。LIPS测定不需要使用放射性同位素或体外转录/翻译，并且是RIP测定在临床水平上的实用替代方案。
To compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2β. Sera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2β. LIPS showed equal sensitivity and specificity to RIP for detecting autoantibodies to GAD65 and IA-2β. Receiver-operating characteristic analysis revealed that the detection of autoantibodies to GAD65 and IA-2β by LIPS and RIP were not statistically different. The LIPS assay does not require the use of radioisotopes or in vitro transcription/translation and is a practical alternative at the clinical level for the RIP assay.