Molecular cloning, expression of Sox5 and its down-regulation of Dmrt1 transcription in zebrafish
Molecular cloning, expression of Sox5 and its down-regulation of Dmrt1 transcription in zebrafish
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DOI：
10.1002/jez.b.21053
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发表时间：
2005-09-15
影响因子：
2.2
通讯作者：
Zhou, RJ
中科院分区：
文献类型：
--
作者：
Gao, S;Zhang, T;Zhou, RJ
doublemex和mab-3相关转录因子1（Dmrt 1）是DM结构域基因家族的创始成员，参与性腺发生。在这里，我们报告了斑马鱼Sox 5的克隆，并使用真实的PCR显示在胚胎发生过程中其表达增加，而Dmrt 1的表达减少。对斑马鱼Dmrt 1近端启动子的鉴定显示了两个正调控区和两个负调控区以及一个Sox 5结合位点。驱动EGFP报告基因的dmrt 1（有或没有Sox结合位点）和Sox 5的共转染进一步显示Sox 5结合Dmrt 1启动子并抑制Dmrt 1表达。Dmrtl和Sox 5之间的这种拮抗伙伴关系表明Dmrtl在早期胚胎发生中的潜在转录调控机制。
The doublesex and mab-3-related transcription factor 1 (Dmrt1) is the founding member of a family of DM domain genes, involved in gonadogenesis. Here we report the cloning of zebrafish Sox5 and use real time PCR to show that its expression increases while expression of Dmrt1 decreases during embryogenesis. Characterization of the proximal promoter of zebrafish Dmrt1 revealed two positive and two negative regulatory regions and a Sox5-binding site. Co-transfection of dmrt1 (with or without the Sox-binding site), driving an EGFP reporter and Sox5 showed further that Sox5 bound the Dmrtl promoter and inhibited Dmrtl expression. This antagonistic partnership between Dmrtl and Sox5 suggests a potential transcriptional regulatory mechanism for Dmrtl in early embryogenesis.