Mouse CD11c+ B220+ Gr1+ plasmacytoid dendritic cells develop independently of the T-cell lineage
Mouse CD11c+ B220+ Gr1+ plasmacytoid dendritic cells develop independently of the T-cell lineage
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DOI：
10.1182/blood-2002-01-0214
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发表时间：
2002-10-15
期刊：
影响因子：
20.3
通讯作者：
MacDonald, HR
中科院分区：
文献类型：
--
作者：
Ferrero, I;Held, W;MacDonald, HR
树突状细胞（DCs）的发育起源存在争议。在小鼠中，CD8α⁺和CD8α⁻ DC亚群通常分别被认为具有淋巴系和髓系起源，尽管在这一点上的证据相互矛盾。最近，一种新的CD11c⁺B220⁺ DC亚群已被鉴定出来，它似乎是产生干扰素α（IFNα）的人类浆细胞样DCs（PDCs）在小鼠中的对应物。我们在此表明，CD11c⁺B220⁺小鼠PDCs像人类PDCs一样，存在于胸腺中，并表达T细胞谱系标志物，如CD8α和CD4。然而，在由Notch - 1或T细胞因子1缺陷小鼠的骨髓细胞建立的混合嵌合体中，PDCs的胸腺内发育可与未成熟T细胞谱系细胞完全分离，Notch - 1或T细胞因子1这两种独立的突变会严重阻碍早期T细胞发育。我们的数据表明，胸腺PDCs并非源自双潜能的T/DC前体细胞。
The developmental origin of dendritic cells (DCs) is controversial. In the mouse CD8alpha(+) and CD8alpha(-) DC subsets are often considered to be of lymphoid and myeloid origin respectively, although evidence on this point is conflicting. Very recently a novel CD11c(+) B220(+) DC subset has been identified that appears to be the murine counterpart to interferon alpha (IFNalpha)-producing human plasmacytoid DCs (PDCs). We show here that CD11c(+) B220(+) mouse PDCs, like human PDCs, are present in the thymus and express T lineage markers such as CD8alpha and CD4. However, the intrathymic development of PDCs can be completely dissociated from immature T lineage cells in mixed chimeras established with bone marrow cells from mice deficient for either Notch-1 or T-cell factor 1, two independent mutations that severely block early T-cell development. Our data indicate that thymic PDCs do not arise from a bipotential T/DC precursor.