Microbial Profiling of Combat Wound Infection through Detection Microarray and Next-Generation Sequencing
Microbial Profiling of Combat Wound Infection through Detection Microarray and Next-Generation Sequencing
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DOI：
10.1128/jcm.00556-14
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发表时间：
2014-07-01
影响因子：
9.4
通讯作者：
Jaing, Crystal J.
中科院分区：
文献类型：
--
作者：
Be, Nicholas A.;Allen, Jonathan E.;Jaing, Crystal J.
战伤的愈合和消退受到常驻微生物植物群的高度影响。因此，我们试图利用新的基因组技术和生物信息学分析来实现伤口中微生物种群的全面检测。我们采用了一种能够检测所有已测序病原体的微阵列，对124例来自美国陆军战斗伤肢体损伤的伤口样本进行了询问。S.服务成员。还通过下一代测序和宏基因组分析处理了一部分样品。阵列分析在51%的所有伤口样本中检测到微生物靶标，其中鲍曼不动杆菌是最常检测到的菌种。在组织活检标本中还检测到多种假单胞菌。不动杆菌质粒pRAY的检测与伤口失败显著相关，而肠道相关细菌的检测与成功愈合显著相关。全基因组测序揭示了样品之间广泛的微生物生物多样性。尽管在治疗过程中观察到时间变化，但总伤口生物负载与伤口结局无显著相关性。鉴于标准微生物学方法不能检测每个伤口中的全部微生物，这些数据强调了补充分子技术以彻底表征伤口相关微生物的重要性。未来应用基因组方案评估微生物含量，可以通过早期和快速识别和管理伤口生物负载的关键模式来应用专门护理。
Combat wound healing and resolution are highly affected by the resident microbial flora. We therefore sought to achieve comprehensive detection of microbial populations in wounds using novel genomic technologies and bioinformatics analyses. We employed a microarray capable of detecting all sequenced pathogens for interrogation of 124 wound samples from extremity injuries in combat-injured U. S. service members. A subset of samples was also processed via next-generation sequencing and metagenomic analysis. Array analysis detected microbial targets in 51% of all wound samples, with Acinetobacter baumannii being the most frequently detected species. Multiple Pseudomonas species were also detected in tissue biopsy specimens. Detection of the Acinetobacter plasmid pRAY correlated significantly with wound failure, while detection of enteric-associated bacteria was associated significantly with successful healing. Whole-genome sequencing revealed broad microbial biodiversity between samples. The total wound bioburden did not associate significantly with wound outcome, although temporal shifts were observed over the course of treatment. Given that standard microbiological methods do not detect the full range of microbes in each wound, these data emphasize the importance of supplementation with molecular techniques for thorough characterization of wound-associated microbes. Future application of genomic protocols for assessing microbial content could allow application of specialized care through early and rapid identification and management of critical patterns in wound bioburden.