Detecting errors in mtDNA data by phylogenetic analysis
Detecting errors in mtDNA data by phylogenetic analysis
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DOI：
10.1007/s004140100228
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发表时间：
2001-10-01
影响因子：
2.1
通讯作者：
Macaulay, V
中科院分区：
文献类型：
--
作者：
Bandelt, HJ;Lahermo, P;Macaulay, V
测序和记录同源DNA片段的样品容易以与生物复制过程相当类似的方式发生复制错误。以前试图获得代表性的mtDNA序列，通常是控制区，用于进化研究或法医学目的，在许多情况下，结果并不令人满意。在给定数据的精确定位问题中，关键因素是在一个由编码区信息支持的已建立的全球系统发育框架内，对密切相关的mtDNA进行系统发育分析。我们开发了一些一般规则，通过这些规则，可以很容易地检测到数据表中可能存在的错误，而无需重复地重读整个序列。遵循这些指导方针，人们可以期望将错误率降低至少一个数量级，尽管在精度上仍然很难击败线粒体伽马聚合酶。
Sequencing and documenting a sample of homologous DNA stretches is prone to copying errors in a way rather analogous to the biological replication process. Previous attempts at obtaining representative mtDNA sequences, typically of the control region, for evolutionary studies or forensic purposes have yielded rather unsatisfactory results in many cases. The key ingredient in pin-pointing problems with given data is the phylogenetic analysis of closely related mtDNAs within the framework of an established worldwide phylogeny that is supported by coding region information. We develop some general rules by which likely errors in data tables can readily be detected without rereading whole sequences repeatedly. Following these guidelines, one can expect to lower the error rate by at least an order of magnitude, although it will still be hard to beat the mitochondrial gamma polymerase in precision.