Sulfide removal and elemental sulfur recycling from a sulfide-polluted medium by Allochromatium vinosum strain 21D.
Sulfide removal and elemental sulfur recycling from a sulfide-polluted medium by Allochromatium vinosum strain 21D.
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发表时间：
2006-12
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通讯作者：
Clemens G. Borkenstein;U. Fischer
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作者：
Clemens G. Borkenstein;U. Fischer
光养紫硫细菌将硫化物氧化为元素硫，元素硫以细胞内硫球的形式储存。含有失活的dsrB基因的突变体葡萄酒异色菌菌株21 D不能进一步将细胞内储存的硫氧化成硫酸盐。该突变体在生物技术过程中用作生物催化剂，以消除合成废水中的硫化物并回收元素硫作为原料。为此目的，突变体在光照的5升生物反应器（30 microE/m2/s PAR）中在30 ℃下在缺氧光养培养基中生长61天。硫化物去除过程是半连续的，由三个连续的补料分批部分组成。硫化物被反复加入到生物反应器中，并被细胞氧化成硫。在突变体的存在下，在硫化物去除过程中没有产生不需要的硫酸盐。实现了49.3 microM/h的最大硫化物去除速率，98.7%的最大硫化物去除效率和60.4%的硫回收。
Phototrophic purple sulfur bacteria oxidize sulfide to elemental sulfur, which is stored as intracellular sulfur globules. The mutant Allochromatium vinosum strain 21D, containing an inactivated dsrB gene, is unable to further oxidize intracellularly stored sulfur to sulfate. This mutant was used as a biocatalyst in a biotechnological process to eliminate sulfide from synthetic wastewater and to recycle elemental sulfur as a raw material. For this purpose, the mutant was grown in an illuminated 5-liter bioreactor (30 microE/m2/s PAR) at 30 degrees C for 61 days in anoxic phototrophic medium. The process of sulfide removal was semi-continuous and consisted of three consecutive fed-batch sections. Sulfide was repeatedly added into the bioreactor and oxidized by the cells to sulfur. In the presence of the mutant, no unwanted sulfate was produced during sulfide removal. A maximum sulfide removal rate of 49.3 microM/h, a maximum sulfide removal efficiency of 98.7%, and 60.4% sulfur recycling were achieved.