Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat
Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat
复制标题
DOI：
10.3791/56807
复制
发表时间：
2018-04-01
影响因子：
1.2
通讯作者：
Zvara, Peter
中科院分区：
文献类型：
--
作者：
Hox, Morten;Mann-Gow, Travis;Zvara, Peter
刺激海绵体神经(CN)和测量海绵体内压(ICP)已被广泛用于测试和评估勃起功能障碍的治疗方法。然而，不同的实验室使用的方法各不相同，陷阱仍然存在。这项研究的目的是描述一种手术技术，它将提供一个可靠的和可重复性的模型。通过暴露坐骨海绵体肌在坐骨结节上的附着点，阴茎脚可以在最小限度地剥离和损伤涉及勃起功能的结构的情况下被插管。用125 Pm双极银电极和生物相容硅胶分离电极-神经复合体，实现了对CN的反复刺激，而不需要举起和干燥。这种方法通过减少神经的拉伸和干燥来预防神经性麻痹，并提供神经的完全隔离，消除漏电，防止对替代路径的刺激。
The stimulation of the cavernous nerve (CN) and measurement of intracavernous pressure (ICP) have been used extensively to test and evaluate therapies for erectile dysfunction. However, the methods used vary between laboratories, and pitfalls still exist. The goal of this study was to describe a surgical technique that would provide a reliable and reproducible model. By exposing the ischiocavernosus muscle at its point of insertion on the ischial tuberosity, the penile crus could be cannulated with minimal dissection and injury to the structures involved in erectile function. Repeated stimulation of the CN, without the need for lifting and drying, was achieved by using a 125 pm bipolar silver electrode and biocompatible silicon glue to isolate the electrode-nerve complex. This method prevents neuropraxia by reducing stretching and drying the nerve and provides complete isolation of the nerve, negating electrical leakage and preventing stimulation of alternative pathways.