THE EFFECTS OF AGING ON THE FUNCTION OF ALVEOLAR MACROPHAGES IN MICE
THE EFFECTS OF AGING ON THE FUNCTION OF ALVEOLAR MACROPHAGES IN MICE
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DOI：
10.1016/0047-6374(93)90024-l
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发表时间：
1993-07-01
影响因子：
5.3
通讯作者：
ORIMO, H
中科院分区：
文献类型：
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作者：
HIGASHIMOTO, Y;FUKUCHI, Y;ORIMO, H
为了确定衰老是否会调节肺泡巨噬细胞（alveolar macrophages， AM）的功能，我们测量了年轻和年老小鼠肺泡巨噬细胞（alveolar macrophages， AM）的tnf - α生成、吞噬功能和表面抗原表达。当AM由ifn - γ（500单位/ml）启动，LPS（100马克/ml）触发时，老年小鼠AM产生的tnf - α明显少于年轻小鼠（年轻小鼠：161.7 +/- 28.2单位/ml；老年小鼠：89.3 +/- 13.6单位/ml， P < 0.05）。老年小鼠AM吞噬乳胶颗粒（1个以上）的比例显著低于年轻小鼠（年轻：78.1 +/- 2.5%，年老：62.8 +/- 3.4%,P < 0.05）。老年小鼠AM中Ia抗原表达量显著高于年轻小鼠，asialo-GM1抗原表达量显著低于年轻小鼠（Ia: young, 0.030 +/- 0.005; old, 0.092 +/- 0.024, P < 0.05; asialo-GM1: young, 0.97 +/- 0.01; old, 0.75 +/- 0.07, P < 0.01）。这些结果表明，小鼠肺泡巨噬细胞功能至少在一定程度上随着衰老而下降。
In order to determine whether the function of alveolar macrophages (AM) is modulated by aging, we measured the TNF-alpha production, phagocytic function, and surface antigen expression of AM from young and old mice. When AM were primed by IFN-gamma (500 units/ml) and triggered by LPS (100 mug/ml), TNF-alpha production by AM was significantly smaller in old mice as compared with young mice (young mice: 161.7 +/- 28.2 units/ml; old mice: 89.3 +/- 13.6 units/ml, P < 0.05). The percentage of AM which phagocytosed latex particles (more than one particle) in old mice was significantly lower than in young mice (young: 78.1 +/- 2.5%; old: 62.8 +/- 3.4%, P < 0.05). Ia antigen expression of the AM was significantly higher and asialo-GM1 antigen expression was significantly lower in old mice than in young mice (Ia: young, 0.030 +/- 0.005; old, 0.092 +/- 0.024, P < 0.05; asialo-GM1: young, 0.97 +/- 0.01; old, 0.75 +/- 0.07, P < 0.01). These results suggest that alveolar macrophage function is at least decreased in part with aging in mice.