Rapid Fabrication of Cell-Laden Alginate Hydrogel 3D Structures by Micro Dip-Coating.
Rapid Fabrication of Cell-Laden Alginate Hydrogel 3D Structures by Micro Dip-Coating.
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DOI：
10.3389/fbioe.2017.00013
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发表时间：
2017
影响因子：
5.7
通讯作者：
Shu W
中科院分区：
文献类型：
--
作者：
Ghanizadeh Tabriz A;Mills CG;Mullins JJ;Davies JA;Shu W
开发一种简单、直接的3D制造方法来3D培养细胞，而不依赖于任何复杂的制造方法，仍然是一个挑战。在本文中，我们描述了一种新技术，该技术允许轻松制造可扩展的3D细胞负载水凝胶结构，而无需复杂的机械：该技术可以仅使用典型细胞生物学实验室中已有的设备完成。该制造方法涉及将覆盖金属棒表面的载有细胞的水凝胶微浸涂到交联剂氯化钙或氯化钡中以形成中空管状结构。该方法可用于形成厚度范围为126至220 µm的单层或多层管状结构。该制造方法使用藻酸盐水凝胶作为主要生物材料，并且可以根据所需的应用添加次要生物材料。我们证明了这种方法的可行性，与生存率超过75%后立即制造和正常反应的细胞在这些管状结构内使用小鼠皮肤胚胎成纤维细胞和人胚肾293细胞含有四环素反应，红色荧光蛋白（tHEK细胞）。
Development of a simple, straightforward 3D fabrication method to culture cells in 3D, without relying on any complex fabrication methods, remains a challenge. In this paper, we describe a new technique that allows fabrication of scalable 3D cell-laden hydrogel structures easily, without complex machinery: the technique can be done using only apparatus already available in a typical cell biology laboratory. The fabrication method involves micro dip-coating of cell-laden hydrogels covering the surface of a metal bar, into the cross-linking reagents calcium chloride or barium chloride to form hollow tubular structures. This method can be used to form single layers with thickness ranging from 126 to 220 µm or multilayered tubular structures. This fabrication method uses alginate hydrogel as the primary biomaterial and a secondary biomaterial can be added depending on the desired application. We demonstrate the feasibility of this method, with survival rate over 75% immediately after fabrication and normal responsiveness of cells within these tubular structures using mouse dermal embryonic fibroblast cells and human embryonic kidney 293 cells containing a tetracycline-responsive, red fluorescent protein (tHEK cells).