Highly sensitive fluorescent detection of dihydroxybenzene based on graphene quantum dots
Highly sensitive fluorescent detection of dihydroxybenzene based on graphene quantum dots
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基于石墨烯量子点的二羟基苯高灵敏荧光检测
DOI：
10.1016/j.snb.2014.08.074
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发表时间：
2014-12-15
影响因子：
8.4
通讯作者：
Tong, Jin
中科院分区：
文献类型：
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作者：
Li, Yongxin;Huang, Hui;Tong, Jin
建立了一种基于荧光石墨烯量子点（GQDs）的超灵敏二羟基苯（DHB）定量检测方法。在H2O2和马萝卜过氧化物酶（HRP）存在下，DHB可氧化生成相应的苯醌，能有效猝灭GQDs的荧光。GQDs的荧光强度比（I/I-0）分别与邻二苯（o-DHB）、间二苯（m-DHB）和对二苯（p-DHB）浓度在0.5 ~ 250 nM、1 ~ 120 nM和0.5 ~ 90 nM范围内成正比。检出限分别为2.0 × 10(-10) mol/L、8.0 × 10(-10) mol/L和3.0 × 10(-10) mol/L，灵敏度明显高于文献报道。此外，该方法可用于雨水和自来水样品中的DHB检测，并可用于检测水溶液中各种可转化为苯醌的分析物。(C) 2014 Elsevier B.V.版权所有
An ultrasensitive dihydroxybenzene (DHB) quantitative detection method was developed based on the fluorescent graphene quantum dots (GQDs). In the presence of H2O2 and horse radish peroxidase (HRP), DHB could be oxidized to the corresponding benzoquinone which could efficiently quench the fluorescence of GQDs. The fluorescence intensity ratio of GQDs (I/I-0) was proportional to the concentration of o-dihydroxybenzene (o-DHB), m-dihydroxybenzene (m-DHB) and p-dihydroxybenzene (p-DHB) in the concentration ranges of 0.5-250, 1-120, and 0.5-90 nM, respectively. The detection limits are 2.0 x 10(-10) mol/L, 8.0 x 10(-10) mol/L and 3.0 x 10(-10) mol/L, which were much more sensitive than previous reports. In addition, the present method could be used for DHB sensing in rainwater and tap-water samples, and had a potential application for detecting various analytes which can be translated into benzoquinone in aqueous solution. (C) 2014 Elsevier B.V. All rights reserved.