Visualization and Documentation of Capsule and Melanin Production in Cryptococcus neoformans.
Visualization and Documentation of Capsule and Melanin Production in Cryptococcus neoformans.
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DOI：
10.1002/cpz1.27
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Nichols CB
中科院分区：
文献类型：
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作者：
Nichols CB
新生隐球菌是一种机会性真菌病原体，主要针对资源丰富和资源有限国家的免疫抑制人群。成功的治疗仅限于少数因隐球菌耐药性而受损的抗真菌药物，从而导致对新药候选药物的密集研究。该物种的两个显著特征是形成多糖壳的能力和真菌细胞的黑化。这些也作为毒力因子，在寄主和环境中保护这种病原体。在这里，我们描述了两种经典的方法来记录胶囊和黑色素。虽然这些方法最初是在几十年前描述和记录的，但由于它们的简单性和成本效益，尽管有更复杂的方法，但它们仍然适用。
Cryptococcus neoformans is an opportunistic fungal pathogen primarily targeting immunosuppressed populations in both resource-rich and resource-limited nations. Successful treatment is limited to a few antifungals that have become compromised by cryptococcal resistance leading to intensive research for new drug candidates. Two distinguishing hallmarks of this species are the ability to develop a polysaccharide capsule and melanization of the fungal cells. These also act as virulence factors, protecting this pathogen in the host as well as in the environment. Here we describe two classic methods to document capsule and melanin. Although initially described and documented over several decades ago, these methods remain relevant in spite of more sophisticated methodology due in part to their simplicity and cost efficiency.