Short-chain fatty acids modify colonic motility through nerves and polypeptide YY release in the rat
Short-chain fatty acids modify colonic motility through nerves and polypeptide YY release in the rat
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DOI：
10.1152/ajpgi.1998.275.6.g1415
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发表时间：
1998-12-01
影响因子：
4.5
通讯作者：
Galimiche, JP
中科院分区：
文献类型：
--
作者：
Cherbut, C;Ferrier, L;Galimiche, JP
短链脂肪酸（SCFAs）被认为是人体大肠内容物的主要阴离子，但其对结肠运动的影响存在争议。本研究探讨SCFAs对大鼠结肠运动的影响及其机制。结肠运动（肌电图）和传输时间（塑料标记物）在清醒的大鼠，而SCFA注入结肠，无论是单独或神经拮抗剂或免疫中和循环多肽YY（PYY）的管理。放免法测定SCFA诱导的PYY释放，然后注入外源性PYY模拟。结肠内输注0.4 mmol/h SCFAs没有影响，而2 mmol/h SCFAs通过降低非推进活动与推进活动的比率降低结肠运动性（36 +/- 3 vs. 57 +/- 4峰丛/h，生理盐水，P < 0.05）。这导致转运率增加（P < 0.01）。无论是α-肾上腺素受体阻滞剂，也没有一氧化氮合酶抑制防止SCFA诱导的运动减少。肠腔内普鲁卡因输注抑制SCFA效应，表明涉及局部神经机制。SCFA结肠输注刺激血液中的PYY释放。循环PYY的免疫中和作用消除了SCFAs对结肠运动的影响，而外源性PYY输注部分再现了这种作用。SCFAs改变大鼠结肠运动模式并增加转运率;局部神经纤维和PYY参与了这种作用。
Short-chain fatty acids (SCFAs) are recognized as the major anions of the large intestinal content in humans, but their effect on colonic motility is controversial. This study explores the colonic motor effect of SCFAs and their mechanisms in the rat. Colonic motility (electromyography) and transit time (plastic markers) were measured in conscious rats while SCFAs were infused into the colon, either alone or after administration of neural antagonists or immunoneutralization of circulating polypeptide YY (PYY). SCFA-induced PYY release was measured by RIA and then simulated by infusing exogenous PYY. Intracolonic infusion of 0.4 mmol/h SCFAs had no effect, whereas 2 mmol/h SCFAs reduced colonic motility (36 +/- 3 vs. 57 +/- 4 spike bursts/h with saline, P < 0.05) by decreasing the ratio of nonpropulsive to propulsive activity. This resulted in an increased transit rate (P < 0.01). Neither alpha-adrenoceptor blockade nor nitric oxide synthase inhibition prevented SCFA-induced motility reduction. Intraluminal procaine infusion suppressed the SCFA effect, indicating that a local neural mechanism was involved. SCFA colonic infusion stimulated PYY release in blood. Immunoneutralization of circulating PYY abolished the effect of SCFAs on colonic motility, whereas exogenous PYY infusion partly reproduced this effect. SCFAs modify colonic motor patterns in the rat and increase transit rate; local nerve fibers and PYY are involved in this effect.