Identification and gene expression profile analysis of a major type of lipoprotein lipase in adult medaka Oryzias latipes
Identification and gene expression profile analysis of a major type of lipoprotein lipase in adult medaka Oryzias latipes
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青鳉成年青鳉脂蛋白脂肪酶主要类型的鉴定及基因表达谱分析
DOI：
10.1007/s12562-014-0826-7
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发表时间：
2015
影响因子：
1.9
通讯作者：
Shugo Watabe and Hideki Ushio
中科院分区：
文献类型：
--
作者：
Lu Wang;Gen Kaneko;Shin-Ichiro Takahashi;Shugo Watabe and Hideki Ushio
脂蛋白脂酶（LPL）在包装在脂蛋白中的循环三酰甘油（TAG）的代谢中起关键作用。鱼类有两个LPL基因，编码LPL 1和LPL 2酶，但其功能尚未明确。为了给进一步的研究提供基础，我们从青鳉成体肝脏中克隆了一个编码LPL的cDNA。发现克隆的LPL基因与几种鱼类的LPL 1基因具有高度同一性，并将其命名为青鳉LPL 1。对公开可用的青鳉表达序列标签（EST）数据库的筛选显示，LPL 1转录丰富的EST从不同的发育阶段和组织。EST筛选还鉴定了第二个LPL样基因，称为青鳉LPL 2，其转录本仅在胚胎期的EST文库中发现。采用3′和5′端快速扩增法获得的LPL 1 cDNA全长为2，259 bp，开放阅读框为1，551 bp，编码516个氨基酸。定量实时PCR进一步证实了LPL 1转录物的普遍分布，在肝脏和内脏脂肪组织中具有高水平，在肠道中具有最低水平。在脑中也发现了相对高的LPL 1基因表达，表明LPL 1在该器官中的潜在功能。
Lipoprotein lipase (LPL) plays a critical role in the metabolism of circulating triacylglycerols (TAGs) packaged in lipoproteins. Fish have two LPL genes, encoding the LPL1 and LPL2 enzymes, but their functions are not yet clearly understood. To provide a basis for further investigation, we cloned a cDNA encoding LPL from the liver of adult medakaOryzias latipes. The cloned LPL gene was found to share a high identity with LPL1 genes from several fish species and was denoted medaka LPL1. Screening against publicly available medaka expressed sequence tag (EST) databases revealed that LPL1 transcripts are abundant in ESTs from various developmental stages and tissues. The EST screening also identified a second LPL-like gene, denoted medaka LPL2, the transcripts of which were found exclusively in EST libraries from embryonic stages. The LPL1 cDNA obtained by a combination of 3′ and 5′ rapid amplification of cDNA ends was 2,259 bp, with an open reading frame of 1,551 bp encoding 516 amino acids. Quantitative real-time PCR further confirmed the ubiquitous distribution of LPL1 transcripts, with high levels in the liver and visceral adipose tissue, and the lowest level in the intestine. A relatively high expression of the LPL1 gene was also found in the brain, suggesting the potential function of LPL1 in this organ.