BLOOD-VESSEL FIBRONECTIN INCREASES IN CONJUNCTION WITH ENDOTHELIAL CELL-PROLIFERATION AND CAPILLARY INGROWTH DURING WOUND-HEALING
BLOOD-VESSEL FIBRONECTIN INCREASES IN CONJUNCTION WITH ENDOTHELIAL CELL-PROLIFERATION AND CAPILLARY INGROWTH DURING WOUND-HEALING
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DOI：
10.1111/1523-1747.ep12500076
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发表时间：
1982-01-01
影响因子：
6.5
通讯作者：
COLVIN, RB
中科院分区：
文献类型：
--
作者：
CLARK, RAF;DELLAPELLE, P;COLVIN, RB
参与伤口愈合的血管生成的调节和血管结构的改变知之甚少。在豚鼠4-mm皮肤伤口的研究中，暴露1-28天，活组织检查并处理1-μ m切片和免疫荧光，血管壁中的纤连蛋白与内皮细胞增殖和毛细血管向内生长一起显著增加。内皮细胞增殖和血管壁纤维连接蛋白的增加都局限于伤口边缘沿着0.5 mm的区域，并发生在伤后3-7天。纤连蛋白很容易在外周肉芽组织的毛细血管中显示，但难以在肉芽组织的中心区域显示，继发于可能归因于成纤维细胞相关纤连蛋白的明亮荧光网状背景染色。随着内皮细胞增殖和毛细血管长入的停止，血管壁中的纤维连接蛋白迅速减少。纤维连接蛋白可能为内皮细胞的有丝分裂和运动提供一个临时的基质。
The regulation of angiogenesis and alterations in the structure of blood vessels taking part in wound healing are poorly understood. In studies of guinea pig 4-mm skin wounds, left uncovered for 1-28 days, biopsied and processed for 1-.mu.m section and immunofluorescence, fibronectin in blood vessel walls markedly increased in conjunction with endothelial cell proliferation and capillary ingrowth. Both the endothelial cell proliferation and the increased vessel wall fibronectin were restricted to a 0.5-mm area along the margin of the wound and occurred 3-7 days after injury. Fibronectin was easily demonstrated in capillaries of the peripheral granulation tissue but was difficult to demonstrate in central areas of the granulation tissue secondary to a brightly fluorescent reticular background staining probably attributable to fibroblast-related fibronectin. The fibronectin in blood vessel walls rapidly diminished as endothelial cell proliferation and capillary ingrowth ceased. Fibronectin may provide a provisional substratum for endothelial cell mitosis and movement.