Isolation of a novel polyomavirus, related to Japanese eel endothelial cell-infecting virus, from marbled eels, Anguilla marmorata (Quoy & Gaimard).
Isolation of a novel polyomavirus, related to Japanese eel endothelial cell-infecting virus, from marbled eels, Anguilla marmorata (Quoy & Gaimard).
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从大理石鳗 Anguilla marmorata (Quoy) 中分离出一种与日本鳗鱼内皮细胞感染病毒相关的新型多瘤病毒
DOI：
10.1111/jfd.12423
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发表时间：
2016
影响因子：
2.5
通讯作者：
F. Nan
中科院分区：
文献类型：
--
作者：
C. Wen;M. Chen;C. S. Wang;P. C. Liu;F. Nan
2012年1月，在台湾养殖的大理石鳗鱼（Anguilla marmorata（Quoy & Gaimard））出现出血和死亡。严重病变的鳗鱼从鳃部流血的，表现为鳃丝中央静脉窦充血，全身出血，类似鳗鱼病毒性内皮细胞坏死。本研究利用从健康黄鳝胸鳍中建立的AMPF细胞系，从患病黄鳝中分离到一种新型多瘤病毒（AmPyV）。发现AmPyV编码一个长T抗原直链基因。系统进化分析表明，AmPyV与日本鳗鲡内皮细胞感染病毒关系密切。PCR检测显示AmPyV感染整个系统器官。AmPyV在25-30 °C的温度下在AMPF、EK-1和EO-2细胞中增殖，子代病毒产量分别为10（7.0）、10（7.4）和10（7.7）TCID 50 mL（-1）。纯化的病毒粒子为二十面体颗粒，直径为70-80 nm。在注射病毒的鳗鱼中未观察到临床症状或死亡率;然而，在注射后2个月，从受感染鳗鱼的脑、眼、肾、鳍和鳃中重新分离出病毒。我们的研究结果表明，AmPyV表现出潜伏感染。其病原有待进一步研究。
Marbled eels, Anguilla marmorata (Quoy & Gaimard), cultured in Taiwan exhibited haemorrhage and mortality in January 2012. The severely diseased eels bled from the gills and showed congestion of the central venous sinus of the gill filaments and haemorrhage throughout the body similar to viral endothelial cell necrosis of eel. In this study, a novel polyomavirus (AmPyV) was isolated from the diseased eels using the AMPF cell line established from the pectoral fin of healthy marbled eels. AmPyV was found to encode a long T-antigen orthologous gene. Phylogenetic analysis showed that AmPyV was closely related to Japanese eel endothelial cell-infecting virus. PCR assays revealed AmPyV infection throughout the systemic organs. AmPyV proliferated in the AMPF, EK-1 and EO-2 cells at temperatures 25-30 °C, and the progeny virus yields were 10(7.0) , 10(7.4) and 10(7.7) TCID50 mL(-1) , respectively. The purified virions were icosahedral particles, 70-80 nm in diameter. No clinical signs or mortality was observed among the eels injected with the virus; however, the virus was reisolated from the brain, eyes, kidneys, fins and gills of infected eels 2 month after injection. Our results suggest that AmPyV exhibits a latent infection. Pathogen of the disease needs to study further.
从显示日本鳗鱼（Anguilla japonica）坏死内皮细胞的样本中分离出新型 DNA 病毒
影响因子：
3.7
作者：
T.Mizutani;S.ono;et al.
通讯作者：
et al.