Fluorescent bionanoprobes to characterize cytoadhesion and cytoinvasion
Fluorescent bionanoprobes to characterize cytoadhesion and cytoinvasion
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DOI：
10.1002/smll.200701086
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发表时间：
2008-05-01
期刊：
影响因子：
13.3
通讯作者：
Gabor, Franz
中科院分区：
文献类型：
--
作者：
Fillafer, Christian;Friedl, Daniela S.;Gabor, Franz
为了克服目前在诊断成像和靶向药物递送方面的局限性，提出了一种高度通用的工具，其可用于代表性地研究用于生物系统的亚微粒的作用。通过将荧光探针BODIPY 4931503（BOD：4，4-二氟-1，3，5，7，8-五甲基-4-硼-3a，4a-二氮杂-s-引达省）稳定连接到可生物降解和生物相容的颗粒核心基质上，开发了一种使胶体可追踪的有效方法。BOD亚微粒被证明是稳定的，可以被表面改性，并表现出高荧光发射。在与人血清白蛋白（非特异性）和麦胚凝集素（生物凝集素）作为模型配体缀合后，发现使用Caco-2细胞的亚微粒的细胞粘附和细胞侵袭特性存在明显差异。这些结果证明了BOD标记的胶体作为生物系统中细胞-颗粒相互作用的多方面研究的通用分析平台的潜力。
To overcome current limitations in diagnostic imaging and targeted drug : delivery, a highly versatile tool is presented that can be used to representatively investigate the effects of submicroparticles intended for the use in biological systems. An effective approach to render colloids trackable is developed by stable attachment of the fluorescent probe BODIPY 4931503 (BOD: 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene) to a biodegradable and biocompatible particle core matrix. BOD submicroparticles are shown to be stable, can be surface modified, and exhibit high fluorescence emission. Upon conjugation with human serum albumin (nonspecific) and wheat germ agglutinin (biorecognitive) as model ligands explicit differences are found in the cytoadhesive and cytoinvasive characteristics of the submicroparticles using Caco-2 cells. These results demonstrate the potency of BOD-labeled colloids as a versatile analytical platform for a multifaceted investigation of cell-particle interactions in biological systems.