PESTICIDE CLASTOGENICITY IN CHINESE-HAMSTER OVARY CELLS
PESTICIDE CLASTOGENICITY IN CHINESE-HAMSTER OVARY CELLS
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DOI：
10.1016/0165-1218(87)90095-4
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发表时间：
1987-07-01
期刊：
影响因子：
--
通讯作者：
WANG, TC
中科院分区：
文献类型：
--
作者：
LIN, MF;WU, CL;WANG, TC
摘要本研究以中国仓鼠卵巢细胞为材料，利用染色体畸变（CAb）诱发法，探讨台湾地区使用最广泛的几种农药--百草枯、甲草胺、丁草胺、甲拌磷及久效磷的致染色体断裂性。二项趋势分析和二项致突变性数据检验的显著性水平是综合判断农药致染色体断裂性的两个标准。除甲拌磷外，在不存在S9体外代谢活化的情况下，所有检测的农药均对CHO细胞具有致染色体断裂作用。使用5 μ l/ml大鼠肝脏提取物S9作为体外代谢活化的来源。添加S9后发现3种不同结局。(1)百草枯：诱导CAb显著减少。（2）久效磷：同时出现细胞毒性降低和致染色体断裂性增加。(3)甲草胺、丁草胺和甲拌磷：细胞毒性增加，但致染色体断裂性无增强迹象。
Paraquat, alachlor, butachlor, phorate and monocrotophos, several of the most extensively used pesticides in Taiwan, were investigated for their clastogenicity using chromosome aberration (CAb) induction in Chinese hamster ovary (CHO) cells. Significance levels of the binomial trend analysis and binomial mutagenicity data test were two criteria for the summary judgement of the pesticide clastogenicity. Except for phorate, all pesticides tested were clastogenic to CHO cells in the absence of in vitro metabolic activation by S9. 5 .mu.l/ml rat-liver extract, S9, were used as the source of in vitro metabolic activation. 3 different outcomes were found after the addition of S9. (1) Paraquat: significant decrease in induced CAbs. (2)Monocrotophos: concomitant occurrence of decreased cytotoxicity and increased clastogenicity. (3) Alachlor, butachlor and phorate: increased cytotoxicities with no sign of enhancement in clastogenicity.