The genetic basis for 3-ADON and 15-ADON trichothecene chemotypes in Fusarium
The genetic basis for 3-ADON and 15-ADON trichothecene chemotypes in Fusarium
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DOI：
10.1016/j.fgb.2011.01.003
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发表时间：
2011-05-01
影响因子：
3
通讯作者：
Proctor, Robert H.
中科院分区：
文献类型：
--
作者：
Alexander, Nancy J.;McCormick, Susan P.;Proctor, Robert H.
某些镰刀菌属物种在世界范围内引起小麦和其他小谷物的赤霉病，并产生镰刀菌烯真菌毒素。这些真菌毒素可引起动物和人类中毒，并可促进某些镰刀菌引起植物疾病的能力。3-乙酰脱氧雪腐镰刀菌烯醇（3-ADON）和15-乙酰脱氧雪腐镰刀菌烯醇（15-ADON）的产生是一些镰刀菌属物种内和之间的重要表型差异。然而，到目前为止，这种化学型差异的遗传基础尚未确定。在这里，我们确定了一致的DNA序列差异，在编码区的生物合成基因TRI 8的3-ADON和15-ADON菌株。对F.禾谷镰刀菌是北美和欧洲小麦赤霉病的主要原因，揭示了来自3-ADON菌株的Tri 8催化三孢霉烯生物合成中间体3，15-二乙酰脱氧雪腐镰刀菌烯醇在碳15处的脱乙酰化以产生3-ADON，而来自15-ADON菌株的Tri 8催化3，15-二乙酰脱氧雪腐镰刀菌烯醇在碳3处的脱乙酰化以产生15-ADON。产生雪腐镰刀菌烯醇的镰刀菌菌株具有与15-ADON菌株中的功能类似的Tri 8。TRI 3，它编码一个蒽碳15乙酰转移酶，被发现在所有三个化学型的功能。总之，我们的数据表明，Tri 8的差异活性决定了镰刀菌属中的3-ADON和15-ADON化学型。爱思唯尔公司出版
Certain Fusarium species cause head blight of wheat and other small grains worldwide and produce trichothecene mycotoxins. These mycotoxins can induce toxicoses in animals and humans and can contribute to the ability of some fusaria to cause plant disease. Production of the trichothecene 3-acetyldeoxynivalenol (3-ADON) versus 15-acetyldeoxynivalenol (15-ADON) is an important phenotypic difference within and among some Fusarium species. However, until now, the genetic basis for this difference in chemotype has not been identified. Here, we identified consistent DNA sequence differences in the coding region of the trichothecene biosynthetic gene TRI8 in 3-ADON and 15-ADON strains. Functional analyses of the TRI8 enzyme (Tri8) in F. graminearum, the predominant cause of wheat head blight in North America and Europe, revealed that Tri8 from 3-ADON strains catalyzes deacetylation of the trichothecene biosynthetic intermediate 3,15-diacetyldeoxynivalenol at carbon 15 to yield 3-ADON, whereas Tri8 from 15-ADON strains catalyzes deacetylation of 3,15-diacetyldeoxynivalenol at carbon 3 to yield 15-ADON. Fusarium strains that produce the trichothecene nivalenol have a Tri8 that functions like that in 15-ADON strains. TRI3, which encodes a trichothecene carbon 15 acetyltransferase, was found to be functional in all three chemotypes. Together, our data indicate that differential activity of Tri8 determines the 3-ADON and 15-ADON chemotypes in Fusarium. Published by Elsevier Inc.