Rmi1 functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1.
Rmi1 functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1.
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Rmi1 通过涉及 Ctf18-RFC 复合物和 Mrc1 的途径在 S 期介导的内聚力建立中发挥作用。
DOI：
10.1016/j.bbrc.2012.09.124
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
and Enomoto T.
中科院分区：
文献类型：
--
作者：
Lai;M.S.;*Seki;M.;Tada;S.;and Enomoto T.
酿酒酵母RecQ解旋酶（Sgs 1）与DNA拓扑异构酶III（Top3）和RecQ介导的基因组不稳定性1（Rmi 1）结合，形成一个进化上保守的复合物，该复合物是处理同源重组中间体和重新启动折叠的复制叉所必需的。以前有报道说，Rmi 1有助于姐妹染色单体的凝聚力，然而，潜在的分子机制一直不清楚。在本研究中，Rmi 1被发现是丰富的区域接近一个早期发射复制起点时，复制叉被逮捕的存在下，在其起源附近。遗传分析表明，Rmi 1促进姐妹染色单体凝聚力的过程中，这是不同的凝聚力建立途径，涉及Ctf 4，Csm 3，Chl 1和途径，涉及Smc 3的乙酰化。另一方面，Rmi 1似乎在涉及Ctf 18-RFC复合物和Mrc 1的通路中起作用，这些复合物和Mrc 1先前被预测调节前导链DNA复制。
Saccharomyces cerevisiae RecQ helicase (Sgs1) combines with DNA topoisomerase III (Top3) and RecQ-mediated genome instability 1 (Rmi1) to form an evolutionarily conserved complex that is required for processing homologous recombination intermediates and restarting collapsed replication forks. It was previously reported that Rmi1 contributes to sister chromatid cohesion; however, the underlying molecular mechanism has been unclear. In the present study, Rmi1 was found to be enriched at the region close to an early-firing replication origin when replication forks were arrested near their origins in the presence of hydroxyurea. Genetic analyses revealed that Rmi1 promoted sister chromatid cohesion in a process that was distinct from both the cohesion establishment pathway involving Ctf4, Csm3, and Chl1 and the pathway involving the acetylation of Smc3. On the other hand, Rmi1 seemed to function in a pathway involving the Ctf18–RFC complex and Mrc1, which were previously predicted to regulate leading-strand DNA replication.