THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE POTEXVIRUS WHITE CLOVER MOSAIC-VIRUS
THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE POTEXVIRUS WHITE CLOVER MOSAIC-VIRUS
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DOI：
10.1093/nar/16.1.291
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发表时间：
1988-01-11
影响因子：
14.9
通讯作者：
GARDNER, RC
中科院分区：
文献类型：
--
作者：
FORSTER, RLS;BEVAN, MW;GARDNER, RC
从一组重叠的cDNA克隆中测定了马铃薯Y病毒白三叶花叶病毒(WC1MV)基因组RNA的完整核苷酸序列(5845个核苷酸)。WC1MV的40个5‘端核苷酸序列与其它痘病毒的5’端序列具有同源性。基因组包含5个开放阅读框，编码MR 147,417、MR 26,356、MR 12,989、MR 7,219和MR 20,684(外壳蛋白)的蛋白质。MR 147,417蛋白具有与其他RNA病毒可能的聚合酶同源的氨基酸序列。MR 26,356和mR 12,989蛋白与大麦条纹花叶病毒RNA.beta蛋白具有同源性。和甜菜坏死黄藤病毒(BNYVV)RNA-2。两种马铃薯Y病毒的柱状包涵体蛋白中也含有部分MR 26,356蛋白。MR 7,219蛋白与BNYVV RNA-3的25K可能的真菌传播因子同源。
The complete nucleotide sequence (5845 nucleotides) of the genomic RNA of the potexvirus white clover mosaic virus (WC1MV) has been determined from a set of overlapping cDNA clones. Forty of the most 5''-terminal nucleotides of WC1MV showed homology to the 5'' sequences of other potexviruses. The genome contained five open reading frames which coded for proteins of Mr 147,417, Mr 26,356, Mr 12,989, Mr 7,219 and Mr 20,684 (the coat protein). The Mr 147,417 protein had domains of amino acid sequence homology with putative polymerases of other RNA viruses. The Mr 26,356 and Mr 12,989 proteins had homology with proteins of the hordeivirus barley stripe mosaic virus RNA.beta. and the furovirus beet necrotic yellow vine virus (BNYVV) RNA-2. A portion of the Mr 26,356 protein was also conserved in the cylindrical inclusion proteins of two potyviruses. The Mr 7,219 protein had homology with the 25K putative fungal transmission factor of BNYVV RNA-3.