Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferation
Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferation
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DOI：
10.1016/j.jds.2012.03.002
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发表时间：
2012-06-01
影响因子：
3.5
通讯作者：
Fujiwara, Mamoru
中科院分区：
文献类型：
--
作者：
Kawasaki, Aya;Hayashi, Yoshihiko;Fujiwara, Mamoru
背景/目的：粘合树脂封闭剂系统通过形成树脂浸渍层来更有效地封闭根管系统。本研究的目的是研究超粘结剂中的洗脱成分对体外培养的成骨细胞增殖的影响。材料和方法：采用生产说明书中的标准粉液比，制成直径5 mm、长10 mm的圆柱形SBS。将树脂块放置在100 mm的培养皿中。成骨细胞以4×10(6)的密度接种于含有10%胎牛血清的最低基本培养液中，并在加湿的培养箱中培养。在有或没有SBS的情况下培养3天后，根据制造商的说明回收和裂解细胞。用丝裂原活化蛋白激酶(MAPK)抗体试验和四甲基偶氮唑盐(3-(4，5-dimethylthyazol-2-yl)-2，5-diphenyltetrazolium)比色法分析洗脱组分诱导的细胞事件。同时，采用电感耦合等离子体发射光谱分析了催化剂组分三正丁基硼烷(TBB)中的硼含量。MU实验结果表明，SBS的成骨成分之一TBB具有促进成骨细胞增殖的作用。含胎牛血清和不含胎牛血清培养的细胞中硼含量分别为1.66+/-0.37和1.74+/-0.30ppm。结论：丹参洗脱组分在体外可促进成骨细胞增殖分化相关MAPKs的表达。洗脱实验和含SBS组分的细胞培养实验均表明，TBB中的硼可能与成骨细胞的增殖激活有关。版权所有(C)2012，中国共和国牙科学会。爱思唯尔台湾有限责任公司出版。版权所有。
Background/purpose: Adhesive resin sealer systems are applied to seal root-canal systems more effectively through the formation of a resin impregnation layer. The purpose of this study was to investigate the effects of eluted components of the adhesive, Super-Bond sealer (SBS), on the proliferation of osteoblastic cells in vitro.Materials and methods: The standard powder:liquid ratio according to the manufacturer's instructions was used to produce a cylindrical block of SBS (5 mm in diameter, 10 mm long) for this elution study. The resin block was placed on a 100-mm culture dish. Osteoblastic cells were seeded at a density of 4 x 10(6) in a-minimum essential medium containing 10% fetal bovine serum and cultured in a humidified incubator. After 3 days of culture with or without SBS, cells were retrieved and lysed according to the manufacturer's instructions. The cellular events induced by the eluted components from SBS were analyzed using an antibody assay for mitogen-activated protein kinases (MAPKs) and a 3-(4,5-dimethylthyazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Furthermore, the concentration of boron, a component of the catalyst, tri-n-butyl borane (TBB), was analyzed using inductively coupled plasma optical emission spectrometry.Results: Expression of MAPKs increased after SBS application. The MU assay indicated that TBB, one of the components of SBS, accelerated the proliferation of osteoblastic cells. Values of boron were 1.66 +/- 0.37 and 1.74 +/- 0.30 ppm in cells cultured with and without FBS, respectively.Conclusions: The eluted components from SBS can increase the expression of some MAPKs related to osteoblastic cell proliferation and differentiation in vitro. Both the elution experiment and treatment of cell culture with SBS components indicated that the boron originating from TBB is likely to be responsible for activation of the proliferation of osteoblastic cells. Copyright (C) 2012, Association for Dental Sciences of the Republic of China. Published by Elsevier Taiwan LLC. All rights reserved.