PATTERNS OF CELL-MOVEMENT WITHIN THE DICTYOSTELIUM SLUG REVEALED BY CELL-TYPE-SPECIFIC, SURFACE LABELING OF LIVING CELLS
PATTERNS OF CELL-MOVEMENT WITHIN THE DICTYOSTELIUM SLUG REVEALED BY CELL-TYPE-SPECIFIC, SURFACE LABELING OF LIVING CELLS
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DOI：
10.1016/0092-8674(94)90053-1
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发表时间：
1994-06-03
期刊：
影响因子：
64.5
通讯作者：
WILLIAMS, J
中科院分区：
文献类型：
--
作者：
ABE, T;EARLY, A;WILLIAMS, J
鳞茎网柄菌体的后部前孢子区有散在分布的细胞，这些细胞具有前柄细胞的许多特性，因此称为类前细胞(ALCS)。通过将编码细胞表面蛋白的基因置于ALC特异性启动子的中心，并对活细胞进行免疫标记，我们分析了ALCS在鼻涕虫内的运动。有一个后方的细胞流动，ALC改变了他们的运动模式，当他们进入柄前区。前柄细胞周期性地从迁移的鼻涕虫中脱落。如果要保持前柄和前孢子鳗鱼的正确比例，就必须替换它们，我们提出了前孢子向前柄细胞转分化的证据，ALCs在转换过程中起到中间作用。因此，无论是在细胞分化方面还是在细胞运动方面，这种鼻涕虫都具有令人惊讶的动态结构。
There are cells scattered in the rear, prespore region of the Dictyostelium slug that share many of the properties of the prestalk cells and that are therefore called anterior-like cells (ALCs). By placing the gene encoding a cell surface protein under the central of an ALC-specific promoter and immunologically labeling the living cells, we analyze the movement of ALCs within the slug. There Is a posterior to anterior cellular flow, and the ALCs change their movement pattern as they enter the prestalk zone. Prestalk cells are periodically shed from the migrating slug. They must be replaced if the correct ratio of prestalk to prespore eels is to be maintained, and we present evidence for the transdifferentiation of prespore into prestalk cells, with ALCs functioning as intermediates in the transition. The slug has, therefore, a surprisingly dynamic structure, both with respect to cellular differentiation and cell movement.