Effect of morphine sulphate eye drops on hyperalgesia in the rat cornea.
Effect of morphine sulphate eye drops on hyperalgesia in the rat cornea.
复制标题
硫酸吗啡滴眼液对大鼠角膜痛觉过敏的影响。
DOI：
10.1016/s0304-3959(03)00260-4
复制
发表时间：
2003
期刊：
影响因子：
7.4
通讯作者：
Honda,NC
中科院分区：
文献类型：
--
作者：
Wenk,NH;Nannenga,NM;Honda,NC
除了它们在中枢介导的镇痛中的传统作用外，阿片类化合物在外周给药时产生显著的效果。使用最近表征的大鼠角膜急性化学损伤模型（Pain（in press）），我们评估了硫酸吗啡滴眼液对角膜炎症和痛觉过敏的影响。局部应用5 μM硫酸吗啡滴眼液制剂以浓度依赖性方式减弱辣椒素诱导的眨眼。然而，吗啡对辣椒素引起的眨眼没有影响，当应用于健康，非炎症大鼠角膜。此外，烧灼后每2小时给予5 μM吗啡可延缓间质水肿和免疫细胞浸润的发展。吗啡的镇痛和抗炎作用均被阿片受体拮抗剂纳洛酮、CTAP和纳曲吲哚阻断。结论：吗啡作用于大鼠角膜μ和δ阿片受体，减轻炎症和痛觉过敏。
In addition to their traditional role in centrally mediated analgesia, opiate compounds produce significant effects when administered peripherally. Using a recently characterized model of acute chemical injury to the rat cornea (Pain (in press)), we assessed the effects of morphine sulphate eye drops on corneal inflammation and hyperalgesia. Topical application of a 5 μM morphine sulphate eye drop preparation attenuated capsaicin-induced blinking in a concentration-dependent manner. However, morphine had no effect on capsaicin-induced blinking when applied to healthy, non-inflamed rat cornea. In addition, 5 μM morphine given every 2 h following cauterization retarded the development of both stromal edema and the infiltration of immune cells. Both the analgesic and anti-inflammatory effects of morphine were prevented by the opioid receptor antagonists naloxone, CTAP, and naltrindole. We conclude that morphine acts on μ and δ opioid receptors located in the rat cornea to attenuate inflammation and hyperalgesia.