Antibiotic susceptibility of Listeria monocytogenes in Denmark 1958-2001
Antibiotic susceptibility of Listeria monocytogenes in Denmark 1958-2001
复制标题
DOI：
10.1111/j.1600-0463.2005.apm1130105.x
复制
发表时间：
2005-01-01
期刊：
影响因子：
2.8
通讯作者：
Bruun, B
中科院分区：
文献类型：
--
作者：
Hansen, JM;Gernep-Smidt, P;Bruun, B
为了了解丹麦单核细胞增生李斯特菌菌株的敏感性是否在多年来发生了变化，我们检查了1958-2001年期间的人类分离株。此外，我们还想比较L。使用纸片扩散试验进行单核细胞增多症敏感性测试，通过E-试验进行MIC测量。主要从血培养和脑脊液中分离的106株菌株与3株参考菌株一起进行了检查。通过E-test和使用Iso-sensitest琼脂的Oxoid纸片检测对以下抗生素的敏感性：青霉素G、氨苄青霉素、美罗培南、庆大霉素、磺胺甲恶唑、甲氧苄啶、环丙沙星、红霉素、万古霉素、利奈唑胺、氯霉素和四环素。除环丙沙星为中等敏感外，两种方法检测的菌株对所有抗生素均主要敏感。然而，对于青霉素，氨苄青霉素和磺胺甲恶唑，虽然纸片扩散试验发现菌株是敏感的，MIC测量通常放置在中间敏感组的敏感性断点以上的菌株一个稀释度。根据最低抑菌浓度（MIC）测定结果，对L.从1958年到2001年，丹麦的单核细胞增多症没有发生变化，在其他地方的人类感染中发现的多重耐药菌株在丹麦也没有发现。
In order to see whether the Susceptibility of Danish Listeria monocytogenes strains has changed over the years we examined a collection of human isolates from the period 1958-2001. We, furthermore, wanted to compare L. monocytogenes susceptibility testing using a disc diffusion assay with MIC measurements performed by the E-test. 106 strains isolated predominantly from blood Cultures and cerebrospinal fluids were examined together with three reference strains. Susceptibility to the following antibiotics was tested by the E-test and by Oxoid discs using Iso-sensitest agar: penicillin G, ampicillin, meropenem, gentamicin, sulphamethoxazole, trimethoprim, ciprofloxacin, erythromycin, vancomycin, linezolid, chloramphenicol and tetracycline. The strains were in the main sensitive to all antibiotics examined using both methods, except for ciprofloxacin, where the strains were intermediate sensitive. However, for penicillin, ampicillin and sulphamethoxazole, while the disc diffusion assay found the strains to be sensitive, MIC measurements generally placed the strains one dilution above the breakpoint for sensitivity in the intermediate sensitive group. Based on the MIC measurements, the antibiotic Susceptibility of L. monocytogenes has not changed in Denmark from 1958 to 2001, and the multiresistant strains found in human infections elsewhere have not been found in Denmark.