Corticotropin-releasing factor and gonadotropin-releasing hormone pulse generator activity in the rhesus monkey. Electrophysiological studies.
Corticotropin-releasing factor and gonadotropin-releasing hormone pulse generator activity in the rhesus monkey. Electrophysiological studies.
复制标题
恒河猴的促肾上腺皮质激素释放因子和促性腺激素释放激素脉冲发生器活性。
DOI：
10.1159/000125563
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发表时间：
1990
影响因子：
4.1
通讯作者：
Knobil,E
中科院分区：
文献类型：
--
作者：
Williams,CL;Nishihara,M;Thalabard,JC;Grosser,PM;Hotchkiss,J;Knobil,E
在6只去势恒河猴的下丘脑内侧基底部植入双侧记录电极阵列，观察了促肾上腺皮质激素释放因子（CRF）对下丘脑促性腺激素释放激素（GnRH）脉冲发生器的电生理作用。在放置在灵长类动物椅子上的动物中记录了6-10小时的多单位活动（MUA）增加的“齐射”。在CRF（200 µg，i. v.）给药前3-4 h，每10 min采集一次血样，通过放射免疫测定法测定LH和皮质醇的循环浓度。并在此后保持3-6小时。CRF导致6只猴子中4只的脉冲发生器活动频率显著降低，MUA截击持续时间显著减少，所有6只猴子的循环皮质醇水平升高。用甲吡酮（30 mg/kg，i.m.）治疗，肾上腺类固醇生成抑制剂，防止CRF引起的血清皮质醇水平的上升，并没有扭转CRF的频率或持续时间的MUA截击的抑制作用。阿片拮抗剂纳洛酮（0.8mg/kg，静脉内，在3只动物中的2只动物中，CRF前10分钟，随后输注0.8 mg/kg/h）阻断了CRF对MUA齐射频率的影响，但未能阻断CRF对MUA齐射持续时间的影响，这表明内源性阿片类药物可能介导CRF对脉冲发生器频率的作用，但对持续时间无影响。
The effect of corticotropin-releasing factor (CRF) on the hypothalamic gonadotropin-releasing hormone (GnRH) pulse generator, the central neuronal system governing pulsatile pituitary luteinizing hormone (LH) secretion, was studied electrophysiologically in 6 ovariectomized rhesus monkeys bearing bilateral arrays of recording elecrodes implanted in the mediobasal hypothalamus. ‘Volleys’ of increased multiunit activity (MUA) were recorded for 6–10 h in animals placed in primate chairs. The circulating concentrations of LH and cortisol were determined by radioimmunoassay in blood samples taken every 10 min for 3–4 h prior to the administration of CRF (200 µg, i.v.) and for 3–6 h thereafter. CRF resulted in a significant decrease in the frequency of pulse generator activity in 4 of 6 animals, a significant decrease in the duration of MUA volleys and a rise in circulating cortisol levels in all 6 monkeys. Treatment with metyrapone (30 mg/kg, i.m.), an inhibitor of adrenal steroidogenesis that prevented the CRF-induced rise in serum cortisol levels, did not reverse the inhibitory effects of CRF on the frequency or duration of MUA volleys. The opiate antagonist naloxone (0.8 mg/kg, i.v., 10 min prior to CRF followed by 0.8 mg/kg/h infusion) blocked the effects of CRF on MUA volley frequency in 2 of 3 animals, but failed to block the effect of CRF on MUA volley duration, suggesting that endogenous opioids may mediate the action of CRF on pulse generator frequency but not on duration.