EVIDENCE OF MELANOGENESIS IN PORCINE RETINAL-PIGMENT EPITHELIAL-CELLS INVITRO
EVIDENCE OF MELANOGENESIS IN PORCINE RETINAL-PIGMENT EPITHELIAL-CELLS INVITRO
复制标题
DOI：
10.1016/0014-4835(90)90003-d
复制
发表时间：
1990-01-01
影响因子：
3.4
通讯作者：
SWART, T
中科院分区：
文献类型：
--
作者：
DOREY, CK;TORRES, X;SWART, T
在猪视网膜色素上皮（RPE）细胞培养物达到融合后数周，出现肉眼可见的棕色病灶。形成病灶的立方细胞含有许多相暗颗粒，这些相暗颗粒不显示脂褐素的自发荧光特征。这里描述的数据表明颗粒是黑素体。电子显微镜检查显示这些细胞中有三种类型的电子致密颗粒：直径为0.3-0.5 μ m的简单球体，直径为1 - 2 μ m的大球体，以及较小球体的溶酶体聚集体。两个球体的基质由40 nm微泡组成，这些微泡也在细胞质中游离并在液泡结构内聚集。RPE细胞及其培养基的反相高效液相色谱检测黑素原，即黑色素生物合成的中间产物，包括几种吲哚衍生物。因此，猪RPE培养物可能是研究黑素生成调控的有用系统。
Several weeks after porcine retinal pigment epithelial (RPE) cell cultures attain confluence, macroscopically visible brown foci appear. The cuboidal cells that form the foci contain numerous phase dark granules that do not exhibit the autofluorescence characteristic of lipofuscin. The data described here indicate that the granules are melanosomes. Electron microscopy revealed three types of electron-dense granules in these cells: simple spheres 0.3-0.5 .mu.m in diameter, large spheres 1 - 2 .mu.m in diameter, and lysosomal aggregations of the smaller spheres. The matrix of both spheres is composed of 40-nm microvesicles that were also found free in the cytoplasm and aggregated within vacuolar structures. Reversed-phase high-performance liquid chromatography of RPE cells and their media detected melanogens, i.e. intermediates of melanin biosynthesis, including several indole derivatives. The porcine RPE cultures therefore may be a useful system for studying melanogenic regulation.