Synthetic hyaluronic acid coating preserves the phenotypes of lymphatic endothelial cells.
Synthetic hyaluronic acid coating preserves the phenotypes of lymphatic endothelial cells.
复制标题
合成透明质酸涂层保留淋巴内皮细胞的表型。
DOI：
10.1039/d3bm00873h
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发表时间：
2023-11-07
影响因子：
6.6
通讯作者：
中科院分区：
文献类型：
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作者：
淋巴管内皮细胞（LECs）在淋巴管系统的形成和维持中起着关键作用，而淋巴管系统对于免疫系统、体液平衡和组织修复至关重要。然而，LEC通常难以在体内研究，并且准确模拟其行为和表型的体外模型有限。特别地，已经显示LEC在体外培养时随着时间的推移失去其淋巴标记物，这反映了它们在体内的可塑性和异质性。由于LEC独特地表达淋巴管内皮透明质酸受体-1（LYVE-1），我们假设透明质酸（HA）表面涂层可以保留LEC的表型和功能。合成具有42%取代度的多巴胺缀合的透明质酸（HA-DP），以使得能够在标准组织培养板上进行表面修饰和缀合。与纤连蛋白涂层和组织培养板对照相比，HA-DP表面涂层能够在体外几次传代中保留淋巴标志物，如Alcohero同源框蛋白1（Prox 1）、podoplanin（PDPN）和LYVE-1。HA-DP培养的LEC表达较低水平的粘着斑激酶（FAK）和雅普/TAZ，这可能是维持淋巴管特性的原因。总的来说，HA-DP涂层可能为体外培养人淋巴管内皮细胞提供一种新的方法，用于基础淋巴生物学和淋巴再生的更具代表性的研究。多巴胺结合透明质酸（HA-DP）表面涂层可以与淋巴管内皮透明质酸受体-1（LYVE-1）相互作用，以保护淋巴管内皮细胞（LEC）的表型和功能。
Lymphatic endothelial cells (LECs) play a critical role in the formation and maintenance of the lymphatic vasculature, which is essential for the immune system, fluid balance, and tissue repair. However, LECs are often difficult to study in vivo and in vitro models that accurately mimic their behaviors and phenotypes are limited. In particular, LECs have been shown to lose their lymphatic markers over time while being cultured in vitro, which reflect their plasticity and heterogeneity in vivo. Since LECs uniquely express lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1), we hypothesized that surface coating with hyaluronic acid (HA) can preserve LEC phenotypes and functionalities. Dopamine conjugated hyaluronic acid (HA–DP) was synthesized with 42% degree of substitution to enable surface modification and conjugation onto standard tissue culture plates. Compared to fibronectin coating and tissue culture plate controls, surface coating with HA–DP was able to preserve lymphatic markers, such as prospero homeobox protein 1 (Prox1), podoplanin (PDPN), and LYVE-1 over several passages in vitro. LECs cultured on HA–DP expressed lower levels of focal adhesion kinase (FAK) and YAP/TAZ, which may be responsible for the maintenance of the lymphatic characteristics. Collectively, the HA–DP coating may provide a novel method for culturing human LECs in vitro toward more representative studies in basic lymphatic biology and lymphatic regeneration. Surface coating with dopamine conjugated hyaluronic acid (HA–DP) can interact with lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) to preserve the phenotypes and functionality of lymphatic endothelial cells (LECs).
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影响因子：
5.3
作者：
Robering JW;Weigand A;Pfuhlmann R;Horch RE;Beier JP;Boos AM
通讯作者：
Boos AM
影响因子：
11.8
作者：
Bazigou, Eleni;Xie, Sherry;Chen, Chun;Weston, Anne;Miura, Naoyuki;Sorokin, Lydia;Adams, Ralf;Muro, Andres F.;Sheppard, Dean;Makinen, Taija
通讯作者：
Makinen, Taija
影响因子：
9.7
作者：
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通讯作者：
Hanjaya-Putra, Donny
影响因子：
14
作者：
通讯作者：
--
影响因子：
20.3
作者：
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通讯作者：
Maurer, Dieter