Activity of Tagetes minuta Linnaeus (Asteraceae) essential oil against L3 Anisakis larvae type 1
Activity of Tagetes minuta Linnaeus (Asteraceae) essential oil against L3 Anisakis larvae type 1
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DOI：
10.1016/j.apjtm.2017.05.005
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发表时间：
2017-05-01
影响因子：
3.1
通讯作者：
Panebianco, Antonio
中科院分区：
文献类型：
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作者：
Giarratana, Filippo;Muscolino, Daniele;Panebianco, Antonio
目的：评价万寿菊的离体药效。精油（TEO）对L3异尖线虫1型幼虫的作用。研究方法：为了评估万寿菊精油对L3异尖线虫幼虫的潜在用途，测试了三种不同的培养基：1）盐水溶液（SS）; 2）工业浸泡溶液; 3）葵花籽油（SO）。对于每种培养基和浓度的TEO（0.1%、0.5%、1.0%和5.0%v/v），将20个寄生虫引入塑料培养皿（直径90mm）中并保持在室温下。作为对照，幼虫在SS、MS和SO中相同的实验条件下保持不含TEO。总共测试了900只幼虫。计算24小时时的标准化平均活力、LT 100、LT 50和灭活百分比。结果：体外试验显示，5%和1%的TEO在盐水溶液中2小时后完全灭活寄生虫。在浸泡溶液中，在使用的所有浓度下4小时后观察到寄生虫完全灭活。在SO中报告了所有TEO浓度的较慢活性。结论：所获得的结果显示出对异尖线虫幼虫的强活性，证实了TEO作为杀幼虫剂用于治疗人类异尖线虫病和工业腌泡过程。
Objective: To evaluate in vitro effects of Tagetes minuta L. essential oil (TEO) on L3 Anisakis larvae type 1. Methods: In order to evaluate the potential use of Tagetes minuta essential oil against L3 Anisakis larvae three different media were tested: 1) a saline solution (SS); 2) an industrial marinating solution; 3) sunflower seeds oil (SO). For each media and concentrations of TEO (0.1%, 0.5%, 1.0% and 5.0% v/v), 20 parasites were introduced into plastic Petri dishes (diameter 90 mm) and maintained at room temperature. As controls, larvae were maintained without TEO under identical experimental conditions in SS, MS and SO. A total of 900 larvae were tested. The normalized mean viability, LT100, LT50 and the percentage of inactivation at 24 hours were calculated. Results: In vitro tests revealed a complete inactivation of parasites in saline solution after 2 hours with 5% and 1% of TEO. In marinating solution, a complete inactivation of parasites was observed after 4 hours at all concentrations used, A slower activity for all TEO concentration was reported in SO. Conclusions: The results obtained, showing a strong activity against Anisakis larvae, confirm TEO as a larvicidal agent in the treatment of human anisakidosis and in the industrial marinating process.