Thermus thermophilus Argonaute Functions in the Completion of DNA Replication.
Thermus thermophilus Argonaute Functions in the Completion of DNA Replication.
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DOI：
10.1016/j.cell.2020.07.036
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发表时间：
2020-09-17
期刊：
影响因子：
64.5
通讯作者：
Zamore PD
中科院分区：
文献类型：
--
作者：
Jolly SM;Gainetdinov I;Jouravleva K;Zhang H;Strittmatter L;Bailey SM;Hendricks GM;Dhabaria A;Ueberheide B;Zamore PD
在许多真核生物中，Argonaute蛋白在短RNA序列的引导下，保护细胞免受转座子和病毒的侵害。在嗜热真细菌中，DNA引导的Argonaute TtAgo防御DNA质粒的转化。在这里，我们报道TtAgo也参与DNA复制。在体内，TtAgo结合来自复制终止的染色体区域的15 - 18nt DNA向导，并与已知在DNA复制中起作用的蛋白质结合。当唯一的嗜热t菌II型拓扑异构酶回转酶被抑制时，TtAgo允许细菌完成其环状基因组的复制。相反，gyrase和TtAgo活性的丧失会减缓生长，并产生长长的香肠状细丝，其中单个细菌通过DNA连接在一起。最后，野生型嗜热t菌战胜了缺乏TtAgo的等基因菌株。我们认为TtAgo的主要作用是帮助嗜热t菌解开DNA复制产生的链结环状染色体。Argonaute蛋白使用短核酸作为向导。在真核生物中，这些包括sirna、mirna和pirna，它们在对抗病毒、调节基因表达和沉默转座子方面的功能已经被广泛研究。Argonaute蛋白在细菌中的功能尚不清楚。Jolly等人报道，在嗜热真细菌（Thermus thermophilus）中，Argonaute从复制末端获得其DNA向导，并参与环状染色体的十烷化，确保细胞成功分裂。嗜热真细菌（Thermus thermophilus）的Argonaute蛋白在来自复制末端的~ 16nt单链DNA的引导下，参与分离DNA复制产生的环状染色体。
In many eukaryotes, Argonaute proteins, guided by short RNA sequences, defend cells against transposons and viruses. In the eubacterium Thermus thermophilus, the DNA-guided Argonaute TtAgo defends against transformation by DNA plasmids. Here, we report that TtAgo also participates in DNA replication. In vivo, TtAgo binds 15–18 nt DNA guides derived from the chromosomal region where replication terminates and associates with proteins known to act in DNA replication. When gyrase, the sole T. thermophilus type II topoisomerase, is inhibited, TtAgo allows the bacterium to finish replicating its circular genome. In contrast, loss of both gyrase and TtAgo activity slows growth and produces long sausage-like filaments in which the individual bacteria are linked by DNA. Finally, wild-type T. thermophilus outcompetes an otherwise isogenic strain lacking TtAgo. We propose that the primary role of TtAgo is to help T. thermophilus disentangle the catenated circular chromosomes generated by DNA replication. Argonaute proteins use short nucleic acids as guides. In eukaryotes, these include siRNAs, miRNAs, and piRNAs, whose functions in fighting viruses, regulating gene expression, and silencing transposons have been extensively studied. The functions of Argonaute proteins in bacteria are less well understood. Jolly et al. report that in the eubacterium Thermus thermophilus, Argonaute derives its DNA guides from the terminus of replication and participates in decatenating circular chromosomes, ensuring successful cell division. Guided by ~16 nt single-stranded DNA from the terminus of replication, the Argonaute protein from the eubacterium Thermus thermophilus participates in separating the circular chromosomes produced by DNA replication.
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作者：
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影响因子：
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发表时间：
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