Lactococcus lactis Anchoring Avian Infectious Bronchitis Virus Multi-Epitope Peptide EpiC Induced Specific Immune Responses in Chickens
Lactococcus lactis Anchoring Avian Infectious Bronchitis Virus Multi-Epitope Peptide EpiC Induced Specific Immune Responses in Chickens
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DOI：
10.1271/bbb.130157
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发表时间：
2013-07-01
影响因子：
1.6
通讯作者：
Yang, Fan
中科院分区：
文献类型：
--
作者：
Cao, Hai-Peng;Wang, Hong-Ning;Yang, Fan
粘膜免疫是预防传染性支气管炎病毒（IBV）感染的关键。为了将病毒抗原安全有效地传递到鸡粘膜免疫系统，我们构建了一株携带IBV多表位基因EpiC的乳酸乳球菌，该基因与金黄色葡萄球菌蛋白a的细胞壁锚定域基因融合，SDS-PAGE和Western blot结果表明，融合肽部分位于细胞表面。重组乳杆菌经口腔和鼻腔接种后，鸡的体液和粘膜免疫反应明显增强，尤其是在鼻腔免疫组。重组乳杆菌鼻免疫组80%的鸡在IBV SAIBk株致死性攻毒后未出现任何临床症状，而非重组乳杆菌鼻免疫组的鸡均表现出明显的典型症状。这些结果表明，锚定IBV抗原的无针重组乳球菌是一种很有希望的抗IBV传播的候选疫苗。
Mucosal immunity is critical in preventing infectious bronchitis virus (IBV) infection. To deliver viral antigens to the mucosal immune system of chickens safely and effectively, we constructed a Lactococcus lactis strain carrying IBV multi-epitope gene EpiC fused with the gene of the cell-wall anchoring domain of Staphylococcus aureus protein A. SDS-PAGE and Western blot results indicated that the fused peptide was located partially on the cell surface. Oral and nasal inoculation with the recombinant L. lactis of chickens elicited significantly high humoral and mucosal immune responses, especially in the nasally immunized group. Eighty percent chickens of the nasally immunized group with recombinant L. lactis did not show any clinical signs after a lethal dose challenge with IBV SAIBk strain, while all the non-recombinant L. lactis immunized chickens exhibited obvious and typical symptoms. These results indicate that needle-free recombinant lactococci anchoring the IBV antigen makes a promising vaccine candidate against the spread of IB.