Effect of blood dilution on recovery of organisms from clinical blood cultures in medium containing sodium polyanethol sulfonate
Effect of blood dilution on recovery of organisms from clinical blood cultures in medium containing sodium polyanethol sulfonate
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血液稀释对聚茴香脑磺酸钠培养基中临床血培养物回收率的影响
DOI：
10.1128/jcm.9.2.248-252.1979
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发表时间：
1979
影响因子：
9.4
通讯作者：
J. Waters
中科院分区：
文献类型：
--
作者：
J. F. Salventi;T. Davies;E. Randall;S. Whitaker;J. Waters
本临床研究旨在评估标准实验室方案，该方案要求血液标本用大于或等于10体积的培养基稀释。采集住院患者血液，分别将1 ml接种到含有2.3、7.3和24 ml BACTEC 6B好氧培养基的3个小瓶中，稀释度分别为1:4、1:10和1:30。这三个测试瓶的处理方式相同，研究在四家医院进行。在接种的2550套小瓶中，174套阳性，105例患者中有临床显著分离株。稀释后24小时（67%）和48小时（90%）的阳性培养数没有差异。这些百分比与BACTEC用户的其他报告一致。每次稀释的阳性瓶数（分别为139、144、147）差异不显著，说明三种稀释的病原微生物回收率相同。尽管总体上是平等的，但两名患者，其中一名接受抗生素治疗，被发现有相关的培养物在1:4的稀释下不能生长。这一发现表明，尽管阳性培养的总体回收率较高，但不能明确推荐1:4的血液稀释。
This clinical study was designed to evaluate the standard laboratory protocol that requires blood specimens be diluted with greater than or equal to 10 volumes of media. Blood was collected from hospitalized patients, and 1 ml was inoculated into each of three vials containing 2.3, 7.3, and 24 ml of BACTEC 6B aerobic medium resulting in dilutions of 1:4, 1:10, and 1:30, respectively. The three test vials were treated identically, and the study was carried out at four hospitals. Of the 2,550 sets of vials inoculated, 174 were positive with clinically significant isolates from 105 patients. There was no difference in the number of positive cultures recovered by 24 h (67%) or 48 h (90%) from any dilution. These percentages agreed with other reports from BACTEC users. The number of positive vials (139, 144, 147, respectively) at each dilution was not significantly different, indicating that all three dilutions showed equal recovery of pathogenic microorganisms. Despite this overall equality, two patients, one on antibiotic therapy, were found to have correlated cultures which failed to grow at the 1:4 dilution. This finding implies that a 1:4 dilution of blood cannot be recommended unequivocably despite the higher overall recovery rate of positive cultures.