Development and birthdates of vasoactive intestinal peptide immunoreactive neurons in the chick proventriculus
Development and birthdates of vasoactive intestinal peptide immunoreactive neurons in the chick proventriculus
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鸡腺胃血管活性肠肽免疫反应神经元的发育和出生日期
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
K. Poulsen
中科院分区：
文献类型：
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作者：
M. Epstein;M. Saffrey;K. Poulsen
为了深入了解调节肠神经系统中递质表型表达的机制，我们通过免疫细胞化学和放射自显影相结合的方法研究了鸡前胃（禽胃的分泌部分）中血管活性肠肽免疫反应（VIP‐IR）肌间神经元的发育和出生日期。检查了鸡胚和鸡的肌间神经丛的整个标本中VIP-IR神经元的出现和数量。我们发现，VIP-IR神经元首先出现在胚胎（E）5.5-6.5天的前胃远端部分。在E7.5，发现单个、成对或小群的VIP-IR神经元，它们与未标记的细胞一起形成原始肌间神经节。在连接神经节的发育中的纤维束内发现了VIP-IR纤维。发现VIP-IR神经元的数量在E15.5胚胎中最大，在4周龄鸡中下降至最大值的68%。
To gain insight into the mechanisms regulating expression of transmitter phenotypes in the enteric nervous system, we have studied the development and birthdate of vasoactive intestinal peptide immunoreactive (VIP‐IR) myenteric neurons in the chicken proventriculus (secretory portion of the avian stomach) by a combination of immunocytochemistry and radioautography. The appearance and numbers of VIP‐IR neurons in whole mounts of the myenteric plexus from chick embryos and chickens were examined. We found that VIP‐IR neurons first appeared at embryonic day (E) 5.5–6.5 in the distal part of the proventriculus. At E7.5, VIP‐IR neurons were found singly, in pairs, or in small groups, which together with unlabeled cells formed primitive myenteric ganglia. VIP‐IR fibers were found within the developing fiber tracts which connected the ganglia. The number of VIP‐IR neurons was found to be maximum in the E15.5 embryo and to decline to 68% of maximum in the 4 week old chicken.