Carbon dots-based ratiometric nanosensor for highly sensitive and selective detection of mercury(II) ions and glutathione
Carbon dots-based ratiometric nanosensor for highly sensitive and selective detection of mercury(II) ions and glutathione
复制标题
基于碳点的比率纳米传感器，用于高灵敏度和选择性检测汞（II）离子和谷胱甘肽
DOI：
10.1039/c6ra21309j
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发表时间：
2016-01-01
期刊：
影响因子：
3.9
通讯作者：
Wu, Yongning
中科院分区：
文献类型：
--
作者：
Lu, Shuaimin;Wu, Di;Wu, Yongning
谷胱甘肽（GSH）在许多细胞过程中起着至关重要的作用。近年来，人们一直致力于开发生物体内GSH传感的有效方法。本文报道了一种以碳量子点（CD）为敏感信号，罗丹明B（Rh B）为参比的比率荧光探针的设计和应用，用于汞离子（Hg 2+）和谷胱甘肽（GSH）的高灵敏检测。所获得的纳米传感器在单一激发波长下显示CD和RhB的特征荧光发射。本实验室首次以柠檬酸钠和组氨酸为原料，采用一锅法合成了功能性环糊精。在Hg ~（2+）存在下，由于Hg ~（2+）与CD表面官能团之间的电子转移，只有CD的荧光被猝灭。随后，由于GSH与Hg ~（2+）有较强的亲和力，加入GSH后，CDs-Hg ~（2+）体系的荧光逐渐恢复，可有效地排除大量的背景干扰。该方法已成功应用于氧化应激模型研究。该方法简单、可靠、灵敏度高、选择性好，在环境监测和生物检测中具有广阔的应用前景。
Glutathione (GSH) plays a crucial role in many cellular processes. Recently, great efforts have been made to develop efficient methods for GSH sensing in organisms. Herein, we reported the design and application of a specific ratiometric fluorescent probe, which contained carbon quantum dots (CDs) as sensing signal and Rhodamine B (RhB) as reference, for highly sensitive detection of mercury ions (Hg2+) and glutathione (GSH). The obtained nanosensor showed characteristic fluorescence emissions of CDs and RhB under a single excitation wavelength. The functional CDs were first synthesized in our laboratory according to a one-pot pyrolysis process of sodium citrate with histidine. In the presence of Hg2+, only the fluorescence of CDs was quenched due to electron transfer between Hg2+ and functional groups on the surface of CDs. Subsequently, the fluorescence of CDs–Hg2+ system was recovered gradually with the addition of GSH due to their stronger affinity with Hg2+, which could effectively exclude numerous background interferences. This method has been successfully applied to oxidative stress model investigation. The proposed ratiometric sensing strategy was proven to be facile, reliable, sensitive and selective, showing great potential for Hg2+ and GSH detection in environmental monitoring and biological detection.