Factors affecting serotonin uptake into human platelets.
Factors affecting serotonin uptake into human platelets.
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影响人体血小板吸收血清素的因素。
DOI：
10.1007/bf00174506
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发表时间：
1988
影响因子：
3.4
通讯作者：
Walker,RF
中科院分区：
文献类型：
--
作者：
Codd,EE;McAllister,TW;Walker,RF
由于血小板5-羟色胺摄取动力学的复杂性，我们研究了一天中的时间、一年中的时间以及受试者的年龄对摄取参数的影响。虽然该分析本身具有很好的重复性，并且摄取5-羟色胺的动力学参数在几天内是稳定的，但在给定的时间内，在个体内，当比较来自一天或一年的不同时间或来自不同个体的样品时，差异相当大。在一组个体的数据中发现Vmax(摩尔/细胞数/时间)和血小板数量之间的负相关关系，这表明Vmax的调节不是在每个细胞的摄取能力水平上，而是以某种方式考虑到受试者血浆中的血小板数量。事实上，以一种新的方式(称为“totalVmax”)表达Vmax，不是基于“每个细胞”或“每个107个细胞”的Vmax，而是基于使用的PRP体积中的血小板总数，大大减少了个体之间和跨时间数据的离散性。虽然Vmax(分子数/细胞数/时间)只显示出随着年龄的减少的趋势，例如，总的Vmax随着受试者的年龄而下降是统计上显著的。与Vmax(摩尔/时间/细胞数)相比，totalVmax(摩尔/时间)可能是一个更符合生理学意义的摄取功能表达方式。
Because of complexities in platelet serotonin uptake dynamics, we studied the influence of the time of day and year as well as the subject's age on uptake parameters. While the assay itself was quite reproducible, and the kinetic parameters of 5 HT uptake were stable over a few days, at a given time, within an individual, the variance was quite large when samples from different times of the day or year or from different individuals were compared. An inverse relationship betweenVmax(moles/cell number/time) and platelet number was found in data from a group of individuals, suggesting regulation ofVmaxnot at the level of uptake capacity per cell, but in a manner that somehow takes into consideration the number of platelets in the subject's plasma. Indeed, expressingVmaxin a new way (called “totalVmax”), not based onVmax“per cell” or “per 107cells” but for the total number of platelets in the volume of PRP used, greatly reduced the scatter in the between-individuals and across-time data. WhileVmax(moles/cell number/time) exhibited only a trend toward reduction with age, for example, the decline in totalVmaxwith subject age was statistically significant. It is suggested that totalVmax(moles/time) may be a more physiologically relevant expression for an uptake function thanVmax(moles/time/cell number).