Maize AKINβγ dimerizes through the KIS/CBM domain and assembles into SnRK1 complexes
Maize AKINβγ dimerizes through the KIS/CBM domain and assembles into SnRK1 complexes
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DOI：
10.1016/j.febslet.2009.05.022
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发表时间：
2009-06-18
期刊：
影响因子：
3.5
通讯作者：
Lumbreras, Victoria
中科院分区：
文献类型：
--
作者：
Lopez-Paz, Cristina;Vilela, Belmiro;Lumbreras, Victoria
SNF1/AMPK/SnRK1 复合物是一种细胞内能量传感器，由三种类型的亚基组成：SnRK1 激酶和两个调节性非催化亚基（指定为 β 和 γ）。我们之前描述过一种非典型植物 γ 亚基，AKIN β γ，它包含一个 N 末端尾部，类似于通常存在于 β 亚基中的所谓 KIS 结构域。然而，尚不清楚 AKIN beta gamma 在体内是否正常与内源性 SnRK1 复合物相关，也不知道其独特的结构域结构如何促进 SnRK1 功能。在这里，我们提供的证据表明，玉米 AKIN beta gamma 是植物细胞中活性 SnRK1 复合物的组成部分。使用互补的方法学方法，我们还表明 AKIN beta gamma 通过由 gamma 和出乎意料的 KIS/CBM 结构域介导的同聚相互作用进行关联。 结构摘要：MINT-7040005：AKIN (uniprotkb: B4FX20) 和 AKIN (uniprotkb: B4FX20) 通过色谱法进行物理相互作用 (MI: 0914) 技术 (MI: 0091)MINT-7039992：AKIN (uniprotkb: B4FX20) 和 AKIN (uniprotkb: B4FX20) 通过双分子荧光互补进行物理相互作用 (MI: 0915) (MI: 0809)MINT-7040024、MINT-7040044、 MINT-7040067：AKIN（uniprotkb：B4FX20）和 AKIN（uniprotkb：B4FX20）通过下拉（MI：0096）绑定（MI：0407）MINT-7039978：SnRK1（uniprotkb：Q8H1L5）和 AKIN（uniprotkb：B4FX20）物理交互 (MI: 0915) 通过双分子荧光互补 (MI: 0809) (C) 2009 欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
The SNF1/AMPK/SnRK1 complex is an intracellular energy sensor composed of three types of subunits: the SnRK1 kinase and two regulatory, non-catalytic subunits (designated beta and gamma). We have previously described an atypical plant gamma-subunit, AKIN beta gamma, which contains an N-terminal tail similar to the so-called KIS domain normally present in beta-subunits. However, it is not known whether AKIN beta gamma normally associates with endogenous SnRK1 complexes in vivo, nor how its unique domain structure might contribute to SnRK1 function. Here, we present evidence that maize AKIN beta gamma is an integral component of active SnRK1 complexes in plant cells. Using complementary methodological approaches, we also show that AKIN beta gamma associates through homomeric interactions mediated by both, the gamma- and, unexpectedly, the KIS/CBM domain.Structured summary:MINT-7040005: AKIN (uniprotkb: B4FX20) and AKIN (uniprotkb: B4FX20) physically interact (MI: 0914) by chromatography technologies (MI: 0091)MINT-7039992: AKIN (uniprotkb: B4FX20) and AKIN (uniprotkb: B4FX20) physically interact (MI: 0915) by bimolecular fluorescence complementation (MI: 0809)MINT-7040024, MINT-7040044, MINT-7040067: AKIN (uniprotkb: B4FX20) and AKIN (uniprotkb: B4FX20) bind (MI: 0407) by pull down (MI: 0096)MINT-7039978: SnRK1 (uniprotkb: Q8H1L5) and AKIN (uniprotkb: B4FX20) physically interact (MI: 0915) by bimolecular fluorescence complementation (MI: 0809) (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.