CHARACTERIZATION AND EXPRESSION OF A CDNA-ENCODING THE HUMAN ANDROGEN RECEPTOR
CHARACTERIZATION AND EXPRESSION OF A CDNA-ENCODING THE HUMAN ANDROGEN RECEPTOR
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DOI：
10.1073/pnas.86.1.327
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发表时间：
1989-01-01
影响因子：
11.1
通讯作者：
MCPHAUL, MJ
中科院分区：
文献类型：
--
作者：
TILLEY, WD;MARCELLI, M;MCPHAUL, MJ
我们报告分离的cDNA编码完整的人雄激素受体。cDNA预测蛋白质的917个氨基酸，分子量为98，918。将cDNA引入异源哺乳动物细胞中引起高水平的蛋白质表达，该蛋白质结合二氢睾酮，具有天然人雄激素受体的亲和力、特异性和沉降特性。与先前克隆的类固醇激素受体的氨基酸序列的比较显示了高度的序列保守性与孕酮，糖皮质激素和盐皮质激素受体在推定的激素和DNA结合域区域。
We report the isolation of a cDNA that encodes the complete human androgen receptor. The cDNA predicts a protein of 917 amino acids with a molecular weight of 98,918. Introduction of the cDNA into heterologous mammalian cells caused expression of high levels of a protein that binds dihydrotestosterone with the affinity, specificity, and sedimentation properties characteristic of the native human androgen receptor. Comparisons with the amino acid sequence of previously cloned steroid hormone receptors revealed a high degree of sequence conservation with the progesterone, glucocorticoid, and mineralocorticoid receptors in the putative hormone and DNA-binding domain regions.