Heat-Induced Production of Human Growth Hormone by High Cell Density Cultivation of Recombinant Escherichia Coli
Heat-Induced Production of Human Growth Hormone by High Cell Density Cultivation of Recombinant Escherichia Coli
复制标题
通过重组大肠杆菌的高细胞密度培养热诱导生产人类生长激素
作者：
F. Tabandeh;S. A. Shojaosadati;A. Zomorodipour;M. Khodabandeh;M. Sanati;B. Yakhchali
用重组大肠杆菌表达了人生长激素基因。大肠杆菌在高细胞密度培养中的作用。在不同的热休克条件下测试人生长激素（hGH）的生产和稳定性。在pH恒定补料分批模式下，在确定培养基和复合培养基中，细胞密度分别为25和60 g l-1，发酵时间从41小时减少到32小时。在复合培养基中以2 g l − 1产生hGH。通过在复合培养基中使用甘油作为主要碳源并采用指数补料，细胞密度和hGH产量分别增加至100 g l − 1和2.7 g l − 1。
The temperature-induced, over-expression of the human growth hormone gene in a recombinant E. coli during high cell density cultivation is reported. Human growth hormone (hGH) production and stability were tested under different heat shock conditions. Cell densities were 25 and 60 g l−1 in a pH-stat fed-batch mode in defined and complex medium, respectively, and the fermentation time was decreased from 41 to 32 h. hGH was produced at 2 g l−1 in complex medium. By using glycerol as main carbon source in the complex medium with exponential feeding, cell density and hGH production were increased to 100 g l−1 and 2.7 g l−1, respectively.
DOI：
10.1073/pnas.82.4.1074
发表时间：
1985-01-01
影响因子：
11.1
作者：
TABOR, S;RICHARDSON, CC
通讯作者：
RICHARDSON, CC