A kinetic study of bitter taste receptor sensing using immobilized porcine taste bud tissues
A kinetic study of bitter taste receptor sensing using immobilized porcine taste bud tissues
复制标题
固定化猪味蕾组织苦味受体传感的动力学研究
DOI：
10.1016/j.bios.2017.01.064
复制
发表时间：
2017
影响因子：
12.6
通讯作者：
Xie Junbo
中科院分区：
文献类型：
--
作者：
Wei Lihui;Qiao Lixin;Pang Guangchang;Xie Junbo
目前，开发一种真实反映味觉组织真实感受的高效检测方法具有重要意义。在这项研究中，首次制造了一种新型生物传感器来研究味蕾组织中感受苦味物质的受体的动力学特征。以猪味蕾组织为传感元件，将夹心式传感膜固定在玻碳电极上组装生物传感器。利用所开发的传感器，测定了蔗糖八乙酸酯、苯甲酸地那铵和槲皮素刺激相应受体引起的响应电流。结果表明，分析者与其受体之间的相互作用符合双曲线（R2=0.9776、0.9980和0.9601），激活常数分别为8.748×10−15mol/L、1.429×10–12mol/L、6.613×10–14mol/L。计算出每个细胞的平均受体数为1.75、28.58和13.23，而信号放大因子分别为1.08×104、2.89×103和9.76×104。这些表明该传感器可用于定量描述细胞或组织受体与其配体的相互作用特征、细胞信号级联的作用、受体的数量以及信号传递途径。
At present, developing an efficient assay method for truly reflecting the real feelings of gustatory tissues is of great importance. In this study, a novel biosensor was fabricated to investigate the kinetic characteristics of the receptors in taste bud tissues sensing bitter substances for the first time. Porcine taste bud tissues were used as the sensing elements, and the sandwich-type sensing membrane was fixed onto a glassy carbon electrode for assembling the biosensor. With the developed sensor, the response currents induced by sucrose octaacetate, denatonium benzoate, and quercetin stimulating corresponding receptors were determined. The results demonstrated that the interaction between the analyst with their receptors were fitting to hyperbola (R2=0.9776, 0.9980 and 0.9601), and the activation constants were 8.748×10−15mol/L, 1.429×10–12mol/L, 6.613×10–14mol/L, respectively. The average number of receptors per cell was calculated as 1.75, 28.58, and 13.23, while the signal amplification factors were 1.08×104, 2.89×103and 9.76×104. These suggest that the sensor can be used to quantitatively describe the interaction characteristics of cells or tissue receptors with their ligands, the role of cellular signaling cascade, the number of receptors, and the signal transmission pathways.