Two novel cyclic hexapeptides from the genetically engineered Actinosynnema pretiosum
Two novel cyclic hexapeptides from the genetically engineered Actinosynnema pretiosum
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来自基因工程 Actinosynnema pretiosum 的两种新型环状六肽
DOI：
10.1007/s00253-016-8017-3
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发表时间：
2017-03-01
影响因子：
5
通讯作者：
Shen, Yuemao
中科院分区：
文献类型：
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作者：
Lu, Chunhua;Xie, Fei;Shen, Yuemao
从来源于Pretiosum辐丝藻ATC 31565的基因工程菌株HGF 052：：asm 18的培养物中分离出两种新型环状六肽，命名为辐丝藻A（1）和B（2），以及三种含色氨酸的二酮哌嗪，即环（L-Trp-L-Trp）（3）、环（L-Trp-N-MeL-Trp）（4）和环（N-MeL-Trp-N-MeL-Trp）（5）。通过光谱分析和单晶X-射线衍射确定了它们的结构。化合物1是含3-氨基-6-羟基-2-哌啶酮的环肽的第一个实例，并且1和2对HeLa和PC 3细胞系显示出中等的细胞毒活性。
Two novel cyclic hexapeptides designated actinosynneptides A (1) and B (2), together with three tryptophan containing diketopiperazines, namely cyclo(L-Trp-L-Trp) (3), cyclo(L-Trp-N-MeL-Trp) (4), and cyclo(N-MeL-Trp-N-MeL-Trp) (5), were isolated from the culture of the genetically engineered strain HGF052::asm18 derived from Actinosynnema pretiosum ATCC31565. Their structures were elucidated on the basis of spectroscopic analyses and single-crystal X-ray diffractions. Compound 1 is the first example of 3-amino-6-hydroxy-2-piperidone-containing cyclic peptides, and 1 and 2 showed moderate cytotoxic activities against HeLa and PC3 cell lines.