RBP-L, a transcription factor related to RBP-J kappa
RBP-L, a transcription factor related to RBP-J kappa
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DOI：
10.1128/mcb.17.5.2679
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发表时间：
1997-05-01
影响因子：
5.3
通讯作者：
Honjo, T
中科院分区：
文献类型：
--
作者：
Minoguchi, S;Taniguchi, Y;Honjo, T
RBP-J κ是一种序列特异性DNA结合蛋白，通过与Notch受体的细胞内区域物理相互作用，在Notch受体下游的信号传导中发挥核心作用。尽管哺乳动物中至少存在四个Notch基因，但尚不清楚每个Notch是否需要特定的下游信号分子。在这里，我们报告的分离和表征的小鼠RBP-J κ相关的基因命名RBP-L，几乎只在肺中表达，相反，无处不在的表达RBP-J κ。为了简单起见，我们建议将RBP-J称为kappa RBP-J。RBP-L蛋白结合到与RBP-J几乎相同的DNA序列上。令人惊讶的是，RBP-L不与已知的四种小鼠Notch蛋白中的任何一种相互作用。虽然我们发现RBP-L和EBNA-2在转录激活中合作，但它们没有显示出可以通过几种体内和体外测定检测到的显著强的蛋白质-蛋白质相互作用。这再次与RBP-J与EBNA-2的物理缔合形成对比。讨论了RBP-L与EBNA-2之间功能相互作用的几种模型。
RBP-J kappa is a sequence-specific DNA binding protein which plays a central role in signalling downstream of the Notch receptor by physically interacting with its intracellular region. Although at least four Notch genes exist in mammals, it is unknown whether each Notch requires a specific downstream signalling molecule. Here we report isolation and characterization of a mouse RBP-J kappa-related gene named RBP-L that is expressed almost exclusively in lung, in contrast to the ubiquitous expression of RBP-J kappa. For simplicity, we propose to call RBP-J kappa RBP-J. The RBP-L protein bound to a DNA sequence almost identical to that of RBP-J. Surprisingly, RBP-L did not interact with any of the known four mouse Notch proteins. Although we found that RBP-L and EBNA-2 cooperated in transcriptional activation, they did not show significantly strong protein-protein interaction that can be detected by several in vivo and in vitro assays. This is again in contrast to physical association of RBP-J with EBNA-2. Several models to explain functional interaction between RBP-L and EBNA-2 are discussed.