Cardiovascular ATIP (Angiotensin receptor type 2 interacting protein) expression in mouse development
Cardiovascular ATIP (Angiotensin receptor type 2 interacting protein) expression in mouse development
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DOI：
10.1002/dvdy.24102
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发表时间：
2014-05
影响因子：
2.5
通讯作者：
K. Bundschu;K. Schuh
中科院分区：
文献类型：
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作者：
K. Bundschu;K. Schuh
背景资料：ATIPs（血管紧张素受体2型[AT 2]相互作用蛋白）被描述为AT 2相互作用蛋白变体，而它们在发育过程中的表达和功能尚不清楚。结果如下：在这里，我们提供了一个详细的ATIP变体在小鼠发育过程中的表达模式，通过可视化Mtus 1启动子活性，Mtus 1 RNA，和随后的ATIP蛋白表达。ATIP在发育中的心血管系统中强烈表达，包括卵黄囊的血管丛和胎盘的胎儿血管部分。此外，ATIP在眼和肢芽发育期间以及在肺和神经系统的后期表达空间上不同。三种鼠ATIP同种型以不同的方式表达，其中同种型1和4与心血管、肺和肢芽发育相关，而同种型3限于脑和眼发育。有趣的是，Mtus 1表达不一定与Agtr 2表达相关，这表明ATIP具有新的但未知的功能，不依赖于AT 2信号传导。结论：ATIP似乎主要参与心血管系统的发育调节，并可能以不同的AT 2依赖性和非依赖性方式发挥作用。因此，这些结果提供了有价值的信息，以进一步阐明不同的功能ATIPs在哺乳动物的发育过程中。发展动力学243：699-711，2014年。© 2013威利期刊公司.
Background: ATIPs (Angiotensin receptor type 2 [AT2] interacting proteins) are described as AT2 interacting protein variants, whereas their expression and functions during development are not known yet. Results: Here, we provide a detailed expression pattern of ATIP variants during mouse development by visualizing Mtus1 promotor activity, Mtus1 RNA, and subsequent ATIP protein expression. ATIPs are strongly expressed in the developing cardiovascular system, including the vascular plexus of the yolk sac and the fetal vascular part of the placenta. Moreover, ATIP is expressed spatially distinct during eye and limb bud development, and in later stages in lung and nervous system. The three murine ATIP isoforms are expressed in a distinct manner, whereupon isoform 1 and 4 are correlated to cardiovascular, lung, and limb bud development and isoform 3 is restricted to brain and eye development. Interestingly, Mtus1 expression is not necessarily correlated to Agtr2 expression, suggesting novel but yet unknown functions for ATIP, independent of AT2 signaling. Conclusions: ATIPs seem to be mainly involved in the developmental regulation of the cardiovascular system and may act in different AT2‐dependent and ‐independent manners. Hence, these results deliver valuable information to further elucidate the different functions of ATIPs in the processes of mammalian development. Developmental Dynamics 243:699–711, 2014. © 2013 Wiley Periodicals, Inc.