Estrogen Attenuates VEGF-initiated Blood-Retina Barrier Breakdown in Male Rats
Estrogen Attenuates VEGF-initiated Blood-Retina Barrier Breakdown in Male Rats
复制标题
DOI：
10.1055/s-0031-1283149
复制
发表时间：
2011-08-01
影响因子：
2.2
通讯作者：
Wei, S. -H.
中科院分区：
文献类型：
--
作者：
Chen, X. -F.;Zhang, M. -N.;Wei, S. -H.
17 beta-Estradiol has been demonstrated to protect blood-brain barrier from disruption and attenuate brain injury in various conditions. The aim of this study was to investigate the effect of 17 beta-estradiol on the blood-retina barrier (BRB) breakdown induced by intravitreous injection of vascular endothelial growth factor (VEGF), a significant mediator of vascular permeability. Intravitreous injection of VEGF was performed to initiate BRB breakdown in male rats with PBS in the contralateral eye as control. 2 doses of 17 beta-estradiol and vehicle control were given to 3 groups of rats. The integrity of the BRB was quantified by Evans blue technique and assessed by fluorescent dyes in retinal sections and wholemounts. BRB breakdown was achieved by VEGF as retinal vascular permeability was increased compared with control eyes (14.66 +/- 4.09 vs. 4.94 +/- 1.20 mu l/g/h, p
