Behavioural characterization and amounts of brain monoamines and their metabolites in mice lacking histamine H1 receptors
Behavioural characterization and amounts of brain monoamines and their metabolites in mice lacking histamine H1 receptors
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DOI：
10.1016/s0306-4522(98)00167-5
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发表时间：
1998-11-01
期刊：
影响因子：
3.3
通讯作者：
Watanabe, T
中科院分区：
文献类型：
--
作者：
Yanai, K;Son, LZ;Watanabe, T
对缺乏组胺H1受体的突变小鼠进行行为评估，以揭示H1受体在小鼠行为中的功能。研究了小鼠在新环境中的探索行为，以发现小鼠中H1受体的缺失是否会影响与其情绪有关的行为。H1受体缺陷小鼠在旷场和活动轮上的Ampectin显着降低。分别采用被动回避反应和高架十字迷宫实验检测患者的认知功能和焦虑水平。被动回避休息的潜伏期没有任何变化。高架十字迷宫实验显示，突变小鼠的转移潜伏期显著延长，表明H1受体与焦虑的控制部分相关。攻击行为进行了检查的居民入侵者的侵略测试。当面对入侵者时，经过6个月的隔离期后，突变小鼠攻击入侵者的速度明显慢于野生型小鼠。分别采用福尔马林试验和强迫游泳试验评价两组的伤害性反应和抑郁或绝望状态。突变小鼠表现出明显的伤害性反应在后期的减少，而不影响早期。强迫游泳试验两组无显著性差异。在H1受体缺失和野生型小鼠中测量单胺及其代谢产物的脑含量。H_1受体缺失小鼠大脑皮层和海马中5-羟色胺的更新率（以5-羟吲哚乙酸和5-羟色胺的比值表示）显著增加，这些结果支持了组胺通过H_1受体调节运动、情绪、记忆和学习、伤害感受和攻击行为等多种神经生理功能的药理学研究结果。(C)1998年IBRO。出版社：Eisevier Science Ltd
Behavioural assessments were made of mutant mice lacking histamine H1 receptors to reveal the function of H1 receptors in the behaviour of mice. Exploratory behaviour of mice in a new environment was examined to discover whether the absence of H1 receptors in mice affects actions relating to their emotions. The H1 receptor-deficient mice showed a significant decrease in ambulation in an open field and on an activity wheel. Cognitive functions and anxiety were examined using passive avoidance response test and the elevated plus-maze test, respectively. The passive avoidance rest did not show any change in latency. The elevated plus-maze test revealed that the transfer latency of the mutant mice was significantly prolonged, indicating that H1 receptors are partly associated with the control of anxiety. Aggressive behaviour was examined by a resident-intruder aggression test. When confronted with an intruder, the mutant mice attacked the intruder significantly slower and less frequently than did wild-type mice after a six-month isolation period. A formalin test and a forced swimming test were used to evaluate the nociceptive response and depressive or despairing state, respectively, of both groups. The mutant mice showed a significant decrease of nociceptive response in the late phase without affecting the early phase. There was no significant difference in the forced swimming test between the two groups. The brain content of monoamines and their metabolites was measured in the H1 receptor null and wild-type mice. The turnover rate of 5-hydroxytryptamine defined by the ratio of 5-hydroxyindoleacetic acid and 5-hydroxytryptamine was significantly increased in the cerebral cortex and hippocampus of H1 receptor null mice.These results support the previous pharmacological findings that histamine modulates various neurophysiological functions such as locomotor activity, emotion, memory and learning, nociception and aggressive behaviour through H1 receptors. (C) 1998 IBRO. Published by Eisevier Science Ltd.