Extracellular ATP functions in alleviating the decrease of PSII photochemistry caused by the infection of Xanthomonas campestris pv. phaseoli
Extracellular ATP functions in alleviating the decrease of PSII photochemistry caused by the infection of Xanthomonas campestris pv. phaseoli
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胞外ATP具有缓解野油菜黄单胞菌感染引起的PSII光化学下降的作用。
DOI：
10.1111/ppa.12473
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发表时间：
2016-06-01
期刊：
影响因子：
2.7
通讯作者：
Zhang, J. -P.
中科院分区：
文献类型：
--
作者：
Jiao, Q. -S.;Feng, H. -Q.;Zhang, J. -P.
细胞外三磷酸腺苷(EATP)可以作为一种信号分子调节广泛的细胞过程。本研究探讨了eATP在菜豆感染野油菜黄单胞菌后叶片组织光化学变化中的作用。菜豆(XCP)。侵染XCP后，叶片的PSⅡ最大光化学效率(Fv/Fm)、光化学最大量子效率(Fv‘/Fm’)、PSⅡ运转效率(ΦPSⅡ)、非循环电子传递速率(ETR)、光化学猝灭(QP)和eATP值均显著降低。同时，非光化学猝灭水平(QN)和调节能量耗散量子效率(Y(NPQ))显著增加。外源ATP浓度为0.2 mM时，对所测各叶绿素荧光参数均无显著影响。但在病叶组织中，外源三磷酸腺苷的应用缓解了Fv/Fm、Fv‘/Fm’、ΦPSⅡ、ETR、qP和eATP水平的下降，并抑制了qN和Y(NPQ)的增加。这些结果表明，病原菌侵染引起的PSII光化学变化可能是由eATP介导的。
Extracellular ATP (eATP) can function as a signalling molecule to regulate a wide range of cellular processes. This present work investigated the role of eATP in mediating the change of PSII (photosystem II) photochemistry of the tissues of Phaseolus vulgaris leaves infected with Xanthomonas campestris pv. phaseoli (Xcp). Infection of the leaves with Xcp caused a significant decrease in the the PSII maximal photochemical efficiency (Fv/Fm), the maximum quantum efficiency of PSII photochemistry at illumination (Fv′/Fm′), the PSII operating efficiency (ΦPSII), the rate of non-cyclic electron transport through PSII (ETR), photochemical quenching (qP), and eATP level in the tissues of the infected leaves. At the same time, the levels of non-photochemical quenching (qN) and the quantum yield of regulated energy dissipation of PSII (Y(NPQ)) were significantly increased. Application of exogenous ATP at 0·2 mm to uninfected leaves had no significant effect on any of the chlorophyll fluorescence parameters being measured. However, in the tissues of infected leaves, the application of exogenous ATP alleviated the decreases of the Fv/Fm, Fv′/Fm′, ΦPSII, ETR, qP and eATP level, and also abolished the increases of qN and Y(NPQ). These results suggest that the change of PSII photochemistry by pathogen infection could be mediated by eATP.