Molecular cloning and characterization of SRAM, a novel insect rel/ankyrin-family protein present in nuclei.
Molecular cloning and characterization of SRAM, a novel insect rel/ankyrin-family protein present in nuclei.
复制标题
SRAM 的分子克隆和表征，SRAM 是一种存在于细胞核中的新型昆虫 rel/锚蛋白家族蛋白。
DOI：
10.1093/oxfordjournals.jbchem.a022708
复制
发表时间：
2000
影响因子：
2.7
通讯作者：
S. Natori
中科院分区：
文献类型：
--
作者：
H. Shiraishi;A. Kobayashi;Y. Sakamoto;T. Nonaka;Y. Mitsui;N. Aozasa;T. Kubo;S. Natori
以前，我们纯化了一个59 kDa的蛋白质，结合到kappaB基序的Sarcophaga凝集素基因。在这里，我们报告它的cDNA克隆和它的一些特性作为一个新的成员的Rel/锚蛋白家族。该蛋白命名为SRAM，含有一个Rel同源结构域、一个核定位信号和4个锚蛋白重复序列，但缺少Relish所含的富丝氨酸结构域和PEST序列。我们发现SRAM定位于NIH-Sape-4细胞的细胞核中，这是一个Sarcophaga的胚胎细胞系。在NIH-Sape-4细胞中，包含kappaB基序的串联重复的麻蝇属凝集素基因启动子被激活。在果蝇mbn-2细胞中，Dif单独激活该报告基因，当SRAM和Dif共转染时检测到协同效应，尽管SRAM单独不激活它。
Previously, we purified a 59-kDa protein that binds to the kappaB motif of the Sarcophaga lectin gene. Here we report its cDNA cloning and some of its characteristics as a novel member of the Rel/Ankyrin-family. This protein, named SRAM, contained a Rel homology domain, a nuclear localization signal and 4 ankyrin repeats, but lacked the Ser-rich domain and PEST sequence that Relish contained. We found that SRAM was localized in the nuclei of NIH-Sape-4 cells, which are an embryonic cell line of Sarcophaga. The Sarcophaga lectin gene promoter containing tandem repeats of the kappaB motifs was activated in NIH-Sape-4 cells. In Drosophila mbn-2 cells, Dif alone activated this reporter gene and a cooperative effect was detected when SRAM and Dif were co-transfected, although SRAM alone did not activate it. This is the first report of a Rel/Ankyrin molecule that exists in the nuclei.