TRANSLATIONAL REPRESSION OF BRLA EXPRESSION PREVENTS PREMATURE DEVELOPMENT IN ASPERGILLUS
TRANSLATIONAL REPRESSION OF BRLA EXPRESSION PREVENTS PREMATURE DEVELOPMENT IN ASPERGILLUS
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DOI：
10.1002/j.1460-2075.1993.tb05899.x
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发表时间：
1993-06-01
期刊：
影响因子：
11.4
通讯作者：
ADAMS, TH
中科院分区：
文献类型：
--
作者：
HAN, ST;NAVARRO, J;ADAMS, TH
构巢曲霉brlA发育调控基因座由两个重叠的转录单位brlA α和brlA β组成，其编码功能相关的多肽。我们使用每个预测的brlA阅读框架和大肠杆菌lacZ基因之间的翻译融合来测试brlA α和brlA β表达的发育调节通过不同机制发生的假设。brlA α是转录控制的，并且大部分brlA α指导的β-半乳糖苷酶活性以brlA依赖性方式调节。相反，brlAbeta mRNA是组成型转录的，但brlA多肽的翻译被存在于brlAbeta mRNA的5'端的短开放阅读框（muORF）的存在所阻止。去除muORF起始密码子导致brlA表达失调，导致发育的不适当激活。我们认为构巢曲霉发育诱导的一个机制涉及翻译控制。
The Aspergillus nidulans brlA developmental regulatory locus consists of two overlapping transcription units, brlAalpha and brlAbeta, which encode functionally related polypeptides. We used translational fusions between each of the predicted brlA reading frames and the Escherichia coli lacZ gene to test the hypothesis that developmental regulation of brlAalpha and brlAbeta expression occurs through different mechanisms. brlAalpha is transcriptionally controlled and a large portion of brlAalpha-directed beta-galactosidase activity is regulated in a brlA-dependent manner. In contrast, brlAbeta mRNA is constitutively transcribed but translation of the brlA polypeptide is prevented by the presence of a short open reading frame (muORF) present in the 5' end of brlAbeta mRNA. Removing the muORF initiation codon leads to deregulated brlA expression, resulting in an inappropriate activation of development. We propose that one mechanism for developmental induction in A.nidulans involves translational control.